KLRK1 Protein (AA 78-216) (Fc-DYKDDDDK Tag)
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- Target See all KLRK1 Proteins
- KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 78-216
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This KLRK1 protein is labelled with Fc-DYKDDDDK Tag.
- Purpose
- Human NKG2D/CD314 Protein
- Sequence
- Phe78-Val216
- Characteristics
- Recombinant Human NKG2D/CD314 Protein is expressed from HEK293 with hFc tag and Flag tag at the N-Terminus.It contains Phe78-Val216.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human MICA, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.28μg/ml determined by ELISA. See testing image for detail.
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Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 78-216) protein (His-Avi Tag)
KLRK1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 73-216) protein (His tag)KLRK1 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) protein (mFc Tag)KLRK1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) protein (His-Avi Tag,Biotin)KLRK1 Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 90-232) protein (His tag)KLRK1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 90-232) protein (Fc Tag)KLRK1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 73-216) (Active) protein (Fc Tag)KLRK1 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 1-216) protein (GST tag)KLRK1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 73-216) (Active) protein (mFc Tag)KLRK1 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 73-216) (Active) protein (His tag)KLRK1 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by reduced SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))
- Alternative Name
- NKG2D (KLRK1 Products)
- Background
- NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
- Molecular Weight
- 43.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
- UniProt
- P26718
- Pathways
- Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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