KLRK1 Protein (AA 99-330) (mFc Tag)

Catalog No. ABIN6961134

This Recombinant KLRK1 protein is produced in HEK-293 Cells.

Quick Overview for KLRK1 Protein (AA 99-330) (mFc Tag) (ABIN6961134)

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 99-330

Purification tag / Conjugate: This KLRK1 protein is labelled with mFc Tag.

Purpose: Recombinant human NKG2D protein with N-terminal mouse Fc

Specificity: MFc(Pro99-Lys330) NKG2D(Ile73-Val216)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Alternative Name: NKG2D

Background: NKG2D,CD314,KLRK1,NK cell receptor D
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. This gene encodes a member of the NKG2 family. The encoded transmembrane protein is characterized by a type II membrane orientation (has an extracellular C terminus) and the presence of a C-type lectin domain. It binds to a diverse family of ligands that include MHC class I chain-related A and B proteins and UL-16 binding proteins, where ligand-receptor interactions can result in the activation of NK and T cells. The surface expression of these ligands is important for the recognition of stressed cells by the immune system, and thus this protein and its ligands are therapeutic targets for the treatment of immune diseases and cancers. Read-through transcription exists between this gene and the upstream KLRC4 (killer cell lectin-like receptor subfamily C, member 4) family member in the same cluster.

Molecular Weight: predicted molecular mass of 42.8 kDa after removal of the signal peptide.The apparent molecular mass of mFc-NKG2D is 50-65 kDa due to glycosylation.

Gene ID: 574240

UniProt: P26718

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process