PLGF Protein (AA 19-221) (Fc Tag)
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- Target See all PLGF (PGF) Proteins
- PLGF (PGF) (Placenta Growth Factor (PGF))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 19-221
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PLGF protein is labelled with Fc Tag.
- Purpose
- Human PGF Protein
- Sequence
- Leu19-Arg221
- Characteristics
- Recombinant Human PGF Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu19-Arg221.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human PGF, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 12.8ng/ml determined by ELISA. See testing image for detail.
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Placenta Growth Factor (PGF) (Active) protein
PGF Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Placenta Growth Factor (PGF) (AA 19-158) protein (His tag)PGF Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Placenta Growth Factor (PGF) (AA 19-158) protein (Fc Tag)PGF Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Placenta Growth Factor (PGF) (AA 19-170) protein (Fc Tag)PGF Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Placenta Growth Factor (PGF) (AA 19-170) (Active) protein (His tag)PGF Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Placenta Growth Factor (PGF) (AA 19-221) (Active) protein (Fc Tag)PGF Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Placenta Growth Factor (PGF) protein (His tag)PGF Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Placenta Growth Factor (PGF) (Active) protein (His tag)PGF Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE Active
Placenta Growth Factor (PGF) (AA 1-170) protein (His-IF2DI Tag)PGF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Placenta Growth Factor (PGF) (AA 19-170) protein (GST tag)PGF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- PLGF (PGF) (Placenta Growth Factor (PGF))
- Alternative Name
- PGF (PGF Products)
- Background
- Placental growth factor (PGF) is another member of the VEGF family of cytokines with pro-angiogenic and pro-inflammatory effects. Retinal inhibition of PGF in combination with VEGF-A prevents vascular leakage and CNV possibly via modulating their own expression in mononuclear phagocytes. PGF-related, optimized strategies to target inflammation-mediated angiogenesis may help to increase efficacy and reduce non-responders in the treatment of wet AMD patients.
- Molecular Weight
- 48.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
- UniProt
- P49763-1
- Pathways
- VEGFR1 Specific Signals
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