SFRP1 Protein (AA 32-314) (His tag)
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- Target See all SFRP1 Proteins
- SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 32-314
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SFRP1 protein is labelled with His tag.
- Purpose
- Human SFRP1/SARP2 Protein
- Sequence
- Ser32-Lys314
- Characteristics
- Recombinant Human SFRP1/SARP2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser32-Lys314.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))
- Alternative Name
- SFRP1 (SFRP1 Products)
- Background
- Secreted frizzled-related protein 1 (SFRP1) is a gene that belongs to the secreted glycoprotein SFRP family. SFRP1 has been classified as a tumor suppressor gene due to the loss of expression in various human cancers, which is mainly attributed by epigenetic inactivation via DNA methylation or transcriptional silencing by microRNAs.
- Molecular Weight
- 33.63 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Tris-Bis PAGE result.
- UniProt
- Q8N474
- Pathways
- WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation
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