MAG Protein (AA 20-516) (His-Avi Tag,Biotin)

Catalog No. ABIN7275610

Product Details

Target: MAG (Myelin Associated Glycoprotein (MAG))

Protein Type: Recombinant

Protein Characteristics: AA 20-516

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This MAG protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human Siglec-4a/MAG Protein

Sequence: Gly20-Pro516

Characteristics: Recombinant Biotinylated Human Siglec-4a/MAG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly20-Pro516.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-Siglec-4a Antibody at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-4a, His Tag with the EC50 of 21.5ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MAG (Myelin Associated Glycoprotein (MAG))

Alternative Name: Siglec-4a (MAG Products)

Background: Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity).

Molecular Weight: 57.6 kDa. Due to glycosylation, the protein migrates to 78-85 kDa based on Tris-Bis PAGE result.

UniProt: P20916

Pathways: Neurotrophin Signaling Pathway