SIGLEC10 Protein (AA 17-546) (Fc-Avi Tag,Biotin)

Catalog No. ABIN7275612

Product Details

Target: SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))

Protein Type: Recombinant

Protein Characteristics: AA 17-546

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SIGLEC10 protein is labelled with Fc-Avi Tag,Biotin.

Purpose: Biotinylated Human Siglec-10 Protein

Sequence: Met17-Thr546

Characteristics: Recombinant Biotinylated Human Siglec-10 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Met17-Thr546.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-Siglec-10 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-10, hFc Tag with the EC50 of 25ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, 500 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))

Alternative Name: Siglec-10 (SIGLEC10 Products)

Background: Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.

Molecular Weight: 85.8 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Tris-Bis PAGE result.

UniProt: Q96LC7-1