SIGLEC10 Protein (AA 17-546) (His tag)
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- Target See all SIGLEC10 Proteins
- SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 17-546
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SIGLEC10 protein is labelled with His tag.
- Purpose
- Human Siglec-10 Protein
- Sequence
- Met17-Thr546
- Characteristics
- Recombinant Human Siglec-10 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met17-Thr546.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Siglec-10, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-Siglec-10 Ab., hFc Tag with the EC50 of 7.4ng/ml determined by ELISA. The affinity constant of 15.9μM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in 20 mM PB, 150 mM NaCl, 25 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))
- Alternative Name
- Siglec-10 (SIGLEC10 Products)
- Synonyms
- PRO940 Protein, SIGLEC-10 Protein, SLG2 Protein, Siglecg Protein, SIGLEC10 Protein, slg2 Protein, pro940 Protein, siglec-10 Protein, sialic acid binding Ig like lectin 10 Protein, sialic acid binding Ig-like lectin 10 Protein, sialic acid-binding Ig-like lectin 10 Protein, SIGLEC10 Protein, Siglec10 Protein, LOC484343 Protein, LOC100066644 Protein, siglec10 Protein, LOC100513863 Protein, LOC100340374 Protein
- Background
- Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
- Molecular Weight
- 59.2 kDa. Due to glycosylation, the protein migrates to 70-79 kDa based on Tris-Bis PAGE result.
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