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SIRPA Protein (AA 31-370) (His tag)

SIRPA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Catalog No. ABIN7275652
  • Target See all SIRPA Proteins
    SIRPA (Signal-Regulatory Protein alpha (SIRPA))
    Protein Type
    Protein Characteristics
    AA 31-370
    • 29
    • 19
    • 5
    • 5
    • 1
    • 34
    • 7
    • 7
    • 6
    • 2
    • 2
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This SIRPA protein is labelled with His tag.
    Human SIRP alpha/CD172a Protein
    Recombinant Human SIRP alpha/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu31-Arg370.
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human SIRP alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 38.1ng/ml determined by ELISA. The affinity constant of 20.6nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Restrictions
    For Research Use only
  • Format
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    -20 °C,-80 °C
    Storage Comment
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    SIRPA (Signal-Regulatory Protein alpha (SIRPA))
    Alternative Name
    SIRP alpha (SIRPA Products)
    BIT Protein, CD172A Protein, MFR Protein, MYD-1 Protein, P84 Protein, PTPNS1 Protein, SHPS1 Protein, SIRP Protein, SIRPA Protein, SIRP-A1 Protein, SIRP-B1 Protein, AI835480 Protein, Bit Protein, CD172a Protein, Ptpns1 Protein, SHP-1 Protein, SHPS-1 Protein, swc3 Protein, signal regulatory protein alpha Protein, myeloperoxidase Protein, signal-regulatory protein alpha Protein, tyrosine-protein phosphatase non-receptor type substrate 1 Protein, SIRPA Protein, MPO Protein, LOC458039 Protein, LOC100400378 Protein, LOC100590224 Protein, Sirpa Protein
    Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
    Molecular Weight
    38.1 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
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