SIRPA Protein (AA 31-370) (hFc Tag)

Catalog No. ABIN7275654

This Recombinant SIRPA protein is produced in HEK-293 Cells.

Quick Overview for SIRPA Protein (AA 31-370) (hFc Tag) (ABIN7275654)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 31-370

Purification tag / Conjugate: This SIRPA protein is labelled with hFc Tag.

Purpose: Human SIRP alpha/CD172a Protein

Sequence: Glu31-Arg370

Characteristics: Recombinant Human SIRP alpha/CD172a Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Glu31-Arg370.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human CD47, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human SIRP alpha, hFc Tag with an affinity constant of 18.9nM as determined in SPR assay (Biacore T200).,Immobilized Human CD47, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 82.1ng/ml determined by ELISA (QC Test).,Immobilized Human SIRP alpha, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 26.9ng/ml determined by ELISA.,Human SIRP alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of SIRP alpha is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: SIRP alpha

Background: Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.

Molecular Weight: 64.1 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Bis-Tris PAGE result.

UniProt: P78324