IL-27 Protein (AA 21-229) (His tag)
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- Target See all IL-27 (IL27) Proteins
- IL-27 (IL27) (Interleukin 27 (IL27))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 21-229
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Origin
- Human
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Source
- CHO Cells
- Purification tag / Conjugate
- This IL-27 protein is labelled with His tag.
- Purpose
- Human IL-27 Protein
- Sequence
- Arg21-Lys229 (IL-27B) & Phe29-Pro243 (IL-27A)
- Specificity
- Uni-Prot: Q14213 (IL-27B), Q8NEV9 (IL-27A)
- Characteristics
- Recombinant Human IL-27 Protein is expressed from CHO with His tag at the C-Terminus.It contains Arg21-Lys229(IL-27B) & Phe29-Pro243(IL-27A).
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test). The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Top Product
- Discover our top product IL27 Protein
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Interleukin 27 (IL27) (AA 1-243) protein (GST tag)
IL27 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 27 (IL27) protein (His tag)IL27 Origin: Mouse Host: Human Cells Recombinant > 90 % as determined by SDS-PAGE
Interleukin 27 (IL27) (AA 177-243) protein (GST tag)IL27 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL-27 (IL27) (Interleukin 27 (IL27))
- Alternative Name
- IL-27 (IL27 Products)
- Background
- Interleukin (IL)-27, a member of the IL-6/IL-12 family, has an important role in modulating inflammation in partnership with innate and adaptive immune cells.
- Molecular Weight
- 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on Tris-Bis PAGE result.
- UniProt
- Q14213
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