SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (Pro13Leu-Mutant) (His tag)

Catalog No. ABIN7490687

Product Details

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Protein Type: Recombinant

Protein Characteristics: Pro13Leu-Mutant

Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Omicron

Source: HEK-293 Cells

Purification tag / Conjugate: This SARS-CoV-2 Nucleocapsid protein is labelled with His tag.

Purpose: Recombinant SARS-CoV-2 Nucleocapsid (P13L, E31del, R32del, S33del, R203K, G204R) protein with C-terminal 6xHis tag

Specificity: Nucleocapsid (P13L, E31del, R32del, S33del, R203K, G204R) (Met1-Ala419) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Alternative Name: SARS-CoV-2 N (SARS-CoV-2 N Products)

Target Type: Viral Protein

Background: Coronavirus contain most of nucleocapsid protein. Coronavirus nucleoproteins (N proteins) localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The nucleolus is the site of ribosome biogenesis and sequesters cell cycle regulatory complexes. Two of the major components of the nucleolus are fibrillarin and nucleolin. These proteins are involved in nucleolar assembly and ribosome biogenesis and act as chaperones for the import of proteins into the nucleolus. Regarding of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is a tool for diagnostic.

Molecular Weight: predicted molecular mass of 46.3 kDa after removal of the signal peptide. The apparent molecular mass of Nucleocapsid (D63G, R203M, D377Y)-His is 55-70 kDa due to glycosylation.

UniProt: P0DTC9