EIF4E Protein (AA 2-217) (GST-His-Tag)
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- Target See all EIF4E Proteins
- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-217
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This EIF4E protein is labelled with GST-His-Tag.
- Sequence
- Ala 2-Val 217
- Characteristics
- A DNA sequence encoding the Human eIF4E protein (P06730) (Ala 2-Val 217) was expressed with a N-GST&C-His tag.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product EIF4E Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Expiry Date
- 12 months
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- Target
- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
- Alternative Name
- eIF4E (EIF4E Products)
- Background
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Abbreviation: eIF4E
Target Synonym: Eukaryotic translation initiation factor 4E,eIF-4E,eIF-4F 25 kDa subunit,mRNA cap-binding protein,EIF4E,EIF4EL1,EIF4F
Background: Eukaryotic translation initiation factor 4E is a 217 amino acids protein that belongs to the eukaryotic initiation factor 4E family. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners.It can recognize and bind the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
- Molecular Weight
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Calculated MW: 48.65 kDa
Observed MW: 52 kDa
- UniProt
- P06730
- Pathways
- BCR Signaling
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