FCGR2A Protein (AA 36-218) (His-Avi Tag,Biotin)

Catalog No. ABIN7536866

Product Details

Target: FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))

Protein Type: Recombinant

Protein Characteristics: AA 36-218

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FCGR2A protein is labelled with His-Avi Tag,Biotin.

Application: SDS-PAGE (SDS), Size-exclusion chromatography-High Pressure Liquid Chromatography (SEC-HPLC), Surface Plasmon Resonance (SPR)

Purpose: Biotinylated human Fc gamma RIIa / CD32a (167H) protein

Sequence: AAPPKAVLKL EPPWINVLQE DSVTLTCQGA RSPESDSIQW FHNGNLIPTH TQPSYRFKAN NNDSGEYTCQ TGQTSLSDPV HLTVLSEWLV LQTPHLEFQE GETIMLRCHS WKDKPLVKVT FFQNGKSQKF SHLDPTFSIP QANHSHSGDY HCTGNIGYTL FSSKPVTITV QVPSMGSSSP MGIGGGLNDI FEAQKIEWHE GGGENLYFQS GGHHHHHHHH HH

Specificity: IgG

Characteristics: The sequence of the extracellular domain of human CD32a (Ala 36-Ile 218) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.
Allotype 167H - the high affinity polymorphic variant for CD32a.

Purification: Nickel and SEC

Purity: > 95 % by SEC-HPLC

Endotoxin Level: <1.0 EU per mg

Biological Activity Comment: Measured by its binding affinity in a SPR assay on a Biacore 8k instrument. Human Fc gamma RIIa / CD32a (167H) protein, immobilized on a CM5 chip via an anti-His antibody, can bind to anti-HER2 human IgG1 (trastuzumab) with an affinity constant (KD) of 1.7 μM.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biotin to protein ratio is confirmed as 0.7-1.0 by the HABA assay. Product has been site-specifically biotinylated using the AVI tag technology, where the lysine residue within the tag is enzymatically labeled with biotin.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: To obtain a final concentration of 1 mg/mL reconstitute 250 μg vials with 250 μL water and 1.0 mg vials with 1.0 mL water. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Do not vortex.

Concentration: 1 mg/mL

Buffer: PBS pH 7.2-7.4 (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4)

Preservative: Without preservative

Storage: RT,4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial.

Expiry Date: 12 months

Target Details

Target: FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))

Alternative Name: CD32a (FCGR2A Products)

Background: CD32A, FCGR2A, FCGRIIA, FCR2A, FCRIIA, IGFR2A, IGFRIIA
Background: Low affinity immunoglobulin gamma Fc receptor IIa, also known as FcγRIIa or CD32a, is a type I integral membrane glycoprotein. CD32a is a member of the immunoglobulin superfamily and is expressed on macrophages, monocytes, neutrophils, eosinophils and dendritic cells, epithelial cells, platelets and activated CD4+ T cells. CD32a binds monomeric IgG with low affinity but is very efficient at binding immune complexes and is involved in phagocytosis and clearing of immune complexes. CD32a is structurally composed of two extracellular immunoglobulin domains of the C2-type that interact with the IgG Fc domain, a transmembrane domain and a short cytoplasmic tail containing the immunoreceptor tyrosine-based activation (ITAM) motif. The product provided only contains the extracellular portion of CD32a. CD32a has two allotypic variants differing at amino acid position 167, one containing histidine (H167) and the other arginine (R167). H167 exhibits a higher affinity to human IgG1 and IgG2 than the R167 does and is thought to be primarily responsible for the phagocytosis of IgG-opsonized bacteria.

Molecular Weight: 24.8 kDa

UniProt: P12318