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FCGR2A Protein (His-Avi Tag,Biotin)

FCGR2A Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Catalog No. ABIN7274284
  • Target See all FCGR2A Proteins
    FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
    Protein Type
    Recombinant
    Origin
    • 25
    • 2
    • 1
    • 1
    • 1
    Human
    Source
    • 17
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This FCGR2A protein is labelled with His-Avi Tag,Biotin.
    Sequence
    Ala36-Ile218(H167)
    Purity
    > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    The affinity constant of 0.680 μM as determined in SPR assay (Biacore T200). See testing image for detail.
    Top Product
    Discover our top product FCGR2A Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
    Storage
    4 °C,-80 °C
    Storage Comment
    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
    Alternative Name
    Fc gamma RIIA (FCGR2A Products)
    Synonyms
    CD32 Protein, CD32A Protein, CDw32 Protein, FCG2 Protein, FCGR2 Protein, FCGR2A1 Protein, FcGR Protein, IGFR2 Protein, CD32a Protein, Fc fragment of IgG receptor IIa Protein, FCGR2A Protein
    Background
    CD32MGC23887, CDw32fc-gamma-RIIa, Fc gamma RIIA, FCG2, Fc-gamma-RIIa, FcGR, FCGR2, FCGR2A, FCGR2A1, FcgRIIA, FCRIIA, fcRII-a, IGFR2MGC30032, IgG Fc receptor II-a, FCG2, CD32A,The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains, these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
    Molecular Weight
    23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
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