ADAM17 Protein (AA 581-642) (mFc Tag)

Catalog No. ABIN7538118

Product Details

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Protein Type: Recombinant

Protein Characteristics: AA 581-642

Origin: Human

Source: Mammalian Cells

Purification tag / Conjugate: This ADAM17 protein is labelled with mFc Tag.

Purpose: Recombinant human ADAM17(581-642) Protein with C-terminal mouse Fc tag

Specificity: ADAM17 (Phe581-Glu642) mFc (Pro99-Lys330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Alternative Name: ADAM17 (ADAM17 Products)

Background: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. The encoded protease functions in the ectodomain shedding of tumor necrosis factor-alpha, in which soluble tumor necrosis factor-alpha is released from the membrane-bound precursor. This protease also functions in the processing of numerous other substrates, including cell adhesion proteins, cytokine and growth factor receptors and epidermal growth factor (EGF) receptor ligands, and plays a prominent role in the activation of the Notch signaling pathway. Elevated expression of this gene has been observed in specific cell types derived from psoriasis, rheumatoid arthritis, multiple sclerosis and Crohn's disease patients, suggesting that the encoded protein may play a role in autoimmune disease. Additionally, this protease may play a role in viral infection through its cleavage of ACE2, the cellular receptor for SARS-CoV and SARS-CoV-2. [provided by RefSeq, Aug 2020]

Molecular Weight: predicted molecular mass of 33.2 kDa after removal of the signal peptide. The apparent molecular mass of ADAM17(581-642)-mFc is 35-55 kDa due to glycosylation.

UniProt: P78536

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus