ZNF143 Protein (AA 1-638) (His tag)
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- Target See all ZNF143 Proteins
- ZNF143 (Zinc Finger Protein 143 (ZNF143))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-638
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ZNF143 protein is labelled with His tag.
- Purpose
- Custom-made recombinant ZNF143 Protein expressed in mammalian cells.
- Sequence
- MLLAQINRDS QGMTEFPGGG MEAQHVTLCL TEAVTVADGD NLENMEGVSL QAVTLADGST AYIQHNSKDA KLIDGQVIQL EDGSAAYVQH VPIPKSTGDS LRLEDGQAVQ LEDGTTAFIH HTSKDSYDQS ALQAVQLEDG TTAYIHHAVQ VPQSDTILAI QADGTVAGLH TGDATIDPDT ISALEQYAAK VSIDGSESVA GTGMIGENEQ EKKMQIVLQG HATRVTAKSQ QSGEKAFRCE YDGCGKLYTT AHHLKVHERS HTGDRPYQCE HAGCGKAFAT GYGLKSHVRT HTGEKPYRCS EDNCTKSFKT SGDLQKHIRT HTGERPFKCP FEGCGRSFTT SNIRKVHVRT HTGERPYYCT EPGCGRAFAS ATNYKNHVRI HTGEKPYVCT VPGCDKRFTE YSSLYKHHVV HTHSKPYNCN HCGKTYKQIS TLAMHKRTAH NDTEPIEEEQ EAFFEPPPGQ GEDVLKGSQI TYVTGVEGDD VVSTQVATVT QSGLSQQVTL ISQDGTQHVN ISQADMQAIG NTITMVTQDG TPITVPAHDA VISSAGTHSV AMVTAEGTEG EQVAIVAQDL AAFHTASSEM GHQQHSHHLV TTETRPLTLV ATSNGTQIAV QLGEQPSLEE AIRIASRIQQ GETPGLDD Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- ZNF143 (Zinc Finger Protein 143 (ZNF143))
- Alternative Name
- ZNF143 (ZNF143 Products)
- Background
- Zinc finger protein 143 (SPH-binding factor) (Selenocysteine tRNA gene transcription-activating factor) (hStaf),FUNCTION: Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8. {ECO:0000269|PubMed:17938208, ECO:0000269|PubMed:9776743}.
- Molecular Weight
- 68.9 kDa
- UniProt
- P52747
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