NAPRT1 Protein (AA 1-538) (His tag)

Catalog No. ABIN7550925

Product Details

Target: NAPRT1 (NAPRT) (Nicotinate phosphoribosyltransferase (NAPRT))

Protein Type: Recombinant

Protein Characteristics: AA 1-538

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This NAPRT1 protein is labelled with His tag.

Purpose: Custom-made recombinant NAPRT Protein expressed in mammalian cells.

Sequence: MAAEQDPEAR AAARPLLTDL YQATMALGYW RAGRARDAAE FELFFRRCPF GGAFALAAGL RDCVRFLRAF RLRDADVQFL ASVLPPDTDP AFFEHLRALD CSEVTVRALP EGSLAFPGVP LLQVSGPLLV VQLLETPLLC LVSYASLVAT NAARLRLIAG PEKRLLEMGL RRAQGPDGGL TASTYSYLGG FDSSSNVLAG QLRGVPVAGT LAHSFVTSFS GSEVPPDPML APAAGEGPGV DLAAKAQVWL EQVCAHLGLG VQEPHPGERA AFVAYALAFP RAFQGLLDTY SVWRSGLPNF LAVALALGEL GYRAVGVRLD SGDLLQQAQE IRKVFRAAAA QFQVPWLESV LIVVSNNIDE EALARLAQEG SEVNVIGIGT SVVTCPQQPS LGGVYKLVAV GGQPRMKLTE DPEKQTLPGS KAAFRLLGSD GSPLMDMLQL AEEPVPQAGQ ELRVWPPGAQ EPCTVRPAQV EPLLRLCLQQ GQLCEPLPSL AESRALAQLS LSRLSPEHRR LRSPAQYQVV LSERLQALVN SLCAGQSP Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NAPRT1 (NAPRT) (Nicotinate phosphoribosyltransferase (NAPRT))

Alternative Name: NAPRT (NAPRT Products)

Background: Nicotinate phosphoribosyltransferase (NAPRTase) (EC 6.3.4.21) (FHA-HIT-interacting protein) (Nicotinate phosphoribosyltransferase domain-containing protein 1),FUNCTION: Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate (PubMed:17604275, PubMed:21742010, PubMed:26042198). Helps prevent cellular oxidative stress via its role in NAD biosynthesis (PubMed:17604275). {ECO:0000269|PubMed:17604275, ECO:0000269|PubMed:21742010, ECO:0000269|PubMed:26042198}.

Molecular Weight: 57.6 kDa

UniProt: Q6XQN6

AlphaFold: Q6XQN6