GK2 Protein (AA 1-554) (His tag)

Catalog No. ABIN7556265

Product Details

Target: GK2 (Glycerol Kinase 2 (GK2))

Protein Type: Recombinant

Protein Characteristics: AA 1-554

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This GK2 protein is labelled with His tag.

Purpose: Custom-made recombinant Gk2 Protein expressed in mammalian cells.

Sequence: MAASKQTSAG PLVGAVVQGT NSTRFLVFNS KTAELVCSHQ VELTQEYPKE GWVEQDPKEI LKSVYECIAK ACEKLAEVNI DISNIKAIGV SNQRETTVVW DKFTGDPLYN AVVWLDLRTQ STVETLTKKI PGNSNFVKSK TGLPLSTYFS AVKLRWMLDN LRPIQKAVEE GRAMFGTIDS WLIWCMTGGV NGGIHCTDVT NACRTMLFNI HSLEWDKDLC DFFEIPMSIL PNVCSSSEIY GLMTSGALEG VPISGCLGDQ SAALVGQMCF HEGQAKNTYG TGCFLLCNTG QKCVFSEHGL LTTLAYKLGK NKPVFYALEG SVAIAGAVIR WLRDNFEIIT TSGEVENLAR EVGTSYGCYF VPAFSGLYAP YWEPSARGII CGLTQFTNKC HIAFAALEAV CFQTREILDA MNRDCGIPLS HLQVDGGMTN NRILMQLQAD ILHIPVVKSV MPETTALGAA MAAGAAEGVN VWSLEPEDLS TILMERYEPQ IQATESEIRF STWKRAVMKS MGWVTAKDPE NGDNPVFSCL PLGFFIVSSM TLLIGARCVS TAEE Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: GK2 (Glycerol Kinase 2 (GK2))

Alternative Name: Gk2 (GK2 Products)

Background: Glycerol kinase 2 (GK 2) (Glycerokinase 2) (EC 2.7.1.30) (ATP:glycerol 3-phosphotransferase 2) (Glycerol kinase, testis specific 2),FUNCTION: Key enzyme in the regulation of glycerol uptake and metabolism. Essential for male fertility and sperm mitochondrial sheath formation (PubMed:28852571, PubMed:30662012). Required for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during spermatogenesis.(PubMed:30662012). Can induce mitochondrial clustering through interactions with PLD6 and up-regulation of phosphatidic acid synthesis in the mitochondria (PubMed:28852571). {ECO:0000269|PubMed:28852571, ECO:0000269|PubMed:30662012}.

Molecular Weight: 60.6 kDa

UniProt: Q9WU65