NDUFA4 Protein (AA 1-82) (Fc Tag)

Catalog No. ABIN7557792

Product Details

Target: NDUFA4 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 4 (NDUFA4))

Protein Type: Recombinant

Protein Characteristics: AA 1-82

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This NDUFA4 protein is labelled with Fc Tag.

Purpose: Custom-made recombinant Ndufa4 Protein expressed in mammalian cells.

Sequence: MLRQILGQAK KHPSLIPLFV FIGAGGTGAA LYVMRLALFN PDVSWDRKNN PEPWNKLGPN EQYKFYSVNV DYSKLKKEGP DF Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NDUFA4 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 4 (NDUFA4))

Alternative Name: Ndufa4 (NDUFA4 Products)

Background: Cytochrome c oxidase subunit NDUFA4,FUNCTION: Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules unsing 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. NDUFA4 is required for complex IV maintenance. {ECO:0000250|UniProtKB:O00483}.

Molecular Weight: 9.3 kDa

UniProt: Q62425

AlphaFold: Q62425