NLRP12 Protein (AA 1-1054) (His tag)

Catalog No. ABIN7562128

Product Details

Target: NLRP12 (NLR Family, Pyrin Domain Containing 12 (NLRP12))

Protein Type: Recombinant

Protein Characteristics: AA 1-1054

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This NLRP12 protein is labelled with His tag.

Purpose: Custom-made recombinant Nlrp12 Protein expressed in mammalian cells.

Sequence: MLPSTARDGL YRLSTYLEEL EAGELKKFKL FLGIAEDLSQ DKIPWGRMEK AGPLEMAQLM VAHMGTREAW LLALSTFQRI HRKDLWERGQ GEDLVRVTPN NGLCLFESQS ACPLDVSPNA PRKDLQTTYK DYVRRKFQLM EDRNARLGEC VNLSNRYTRL LLVKEHSNPI WTQQKFVDVE WERSRTRRHQ TSPIQMETLF EPDEERPEPP HTVVLQGAAG MGKSMLAHKV MLDWADGRLF QGRFDYVFYI SCRELNRSHT QCSVQDLISS CWPERGISLE DLMQAPDRLL FIIDGFDKLH PSFHDAQGPW CLCWEEKQPT EVLLGSLIRR LLLPQVSLLI TTRPCALEKL HGLLEHPRHV EILGFSEEAR KEYFYRYFHN TGQASRVLSF LMDYEPLFTM CFVPMVSWVV CTCLKQQLES GELLRQTPRT TTAVYMFYLL SLMQPKPGTP TFKVPANQRG LVSLAAEGLW NQKILFDEQD LGKHGLDGAD VSTFLNVNIF QKGIKCEKFY SFIHLSFQEF FAAMYCALNG REAVRRALAE YGFSERNFLA LTVHFLFGLL NEEMRCYLER NLGWSISPQV KEEVLAWIQN KAGSEGSTLQ HGSLELLSCL YEVQEEDFIQ QALSHFQVVV VRSISTKMEH MVCSFCARYC RSTEVLHLHG SAYSTGMEDD PPEPSGVQTQ STYLQERNML PDVYSAYLSA AVCTNSNLIE LALYRNALGS QGVRLLCQGL RHASCKLQNL RLKRCQISGS ACQDLAAAVI ANRNLIRLDL SDNSIGVPGL ELLCEGLQHP RCRLQMIQLR KCLLEAAAGR SLASVLSNNS YLVELDLTGN PLEDSGLKLL CQGLRHPVCR LRTLWLKICH LGQASCEDLA STLKMNQSLL ELDLGLNDLG DSGVLLLCEG LSHPDCKLQT LRLGICRLGS VACVGIASVL QVNTCLQELD LSFNDLGDRG LQLLGEGLRH QTCRLQKLWL DNCGLTSKAC EDLSSILGIS QTLHELYLTN NALGDTGVCL LCKRLRHPGC KLRVLWLFGM DLNKKTHRRM AALRVTKPYL DIGC Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NLRP12 (NLR Family, Pyrin Domain Containing 12 (NLRP12))

Alternative Name: Nlrp12 (NLRP12 Products)

Background: NACHT, LRR and PYD domains-containing protein 12 (Monarch-1) (PYRIN-containing APAF1-like protein 7) (PYPAF7),FUNCTION: Plays an essential role as an potent mitigator of inflammation (PubMed:26521018, PubMed:30559449). Primarily expressed in dendritic cells and macrophages, inhibits both canonical and non-canonical NF-kappa-B and ERK activation pathways (PubMed:30559449). Functions as a negative regulator of NOD2 by targeting it to degradation via the proteasome pathway (PubMed:30559449). In turn, promotes bacterial tolerance (PubMed:30559449). Inhibits also the RIGI-mediated immune signaling against RNA viruses by reducing the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked RIGI activation but enhancing the E3 ubiquitin ligase RNF125-mediated 'Lys-48'-linked RIGI degradation (By similarity). Acts also as a negative regulator of inflammatory response to mitigate obesity and obesity-associated diseases in adipose tissue (PubMed:30212649). {ECO:0000250|UniProtKB:P59046, ECO:0000269|PubMed:26521018, ECO:0000269|PubMed:30212649, ECO:0000269|PubMed:30559449}.

Molecular Weight: 119.3 kDa

UniProt: E9Q5R7

AlphaFold: E9Q5R7

Pathways: Positive Regulation of Endopeptidase Activity, Inflammasome