POLE2 Protein (AA 1-527) (His tag)
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- Target See all POLE2 Proteins
- POLE2 (Polymerase (DNA Directed), epsilon 2 (p59 Subunit) (POLE2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-527
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This POLE2 protein is labelled with His tag.
- Purpose
- Made-to-order recombinant Pole2 Protein expressed in mammalian cells.
- Sequence
- MAPERLRSRT VSAFKLRGLL LRSEATKYLT EALQSVTELE LEENLGKIID AVEKQPLSSN MIERSVVEAA VQECSQSMDE TIEHVFNIIG AFDIPRFIYN SERKKFLPLS MTNHAAPTLF GTARDKAELY LERYTILHQR THRHELFTPP VIGSHLEESG SKFQLKTIET LLGSTSKVGD VIVLGMITQL KEGKFFLEDP TGTVQLDLSK AQFHSGLYTE ACFVLAEGWF EDQVFHANAF GFPPTEPSST TRAYYGNINF FGGPSNASVK TYTKLRQLED ENKDAMFVIV SDVWLDQVQV LEKFHVMFSG YSPAPPTCFI LCGNFSSAPY GKNQVQALKD SLKSLADIIC EYPSIHQSSR FVFVPGPEDP GFGSILPRPP LAESITQEFR QRVPFSVFTT NPCRIQYCTQ EIIIFREDLV NKMCRNCVRF PSSSLDIPTH FVKTILSQGH LAPLPLYVCP VHWAYDYTLR VYPVPDLLVI ADKYDPFTVT NTDCLCINPG SFPRSGFAFK VFYPSSKTVE DSKLQGF Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
- Top Product
- Discover our top product POLE2 Protein
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Polymerase (DNA Directed), epsilon 2 (p59 Subunit) (POLE2) (AA 1-527) protein (His tag)
custom-made POLE2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Polymerase (DNA Directed), epsilon 2 (p59 Subunit) (POLE2) (AA 1-100) protein (GST tag)POLE2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polymerase (DNA Directed), epsilon 2 (p59 Subunit) (POLE2) protein (Myc-DYKDDDDK Tag)POLE2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- POLE2 (Polymerase (DNA Directed), epsilon 2 (p59 Subunit) (POLE2))
- Alternative Name
- Pole2 (POLE2 Products)
- Background
- DNA polymerase epsilon subunit 2 (DNA polymerase II subunit 2) (DNA polymerase epsilon subunit B),FUNCTION: Accessory component of the DNA polymerase epsilon complex (By similarity). Participates in DNA repair and in chromosomal DNA replication (By similarity). {ECO:0000250|UniProtKB:P24482, ECO:0000250|UniProtKB:P56282}.
- Molecular Weight
- 59.4 kDa
- UniProt
- O54956
- AlphaFold
- O54956
- Pathways
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
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