SNX27 Protein (AA 1-539) (His tag)

Catalog No. ABIN7563717

Product Details

Target: SNX27 (Sorting Nexin 27 (SNX27))

Protein Type: Recombinant

Protein Characteristics: AA 1-539

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This SNX27 protein is labelled with His tag.

Purpose: Custom-made recombinant Snx27 Protein expressed in mammalian cells.

Sequence: MADEDGEGIH PSAPHRNGGG GGGSGLHCAG NGGGGGGGPR VVRIVKSESG YGFNVRGQVS EGGQLRSING ELYAPLQHVS AVLPGGAADR AGVRKGDRIL EVNGVNVEGA THKQVVDLIR AGEKELILTV LSVPPHEADN LDPSDDSLGQ SFYDYTEKQA VPISVPTYKH VEQNGEKFVV YNVYMAGRQL CSKRYREFAI LHQNLKREFA NFTFPRLPGK WPFSLSEQQL DARRRGLEEY LEKVCSIRVI GESDIMQEFL SESDENYNGV SDVELRVALP DGTTVTVRVK KNSTTDQVYQ AIAAKVGMDS TTVNYFALFE VINHSFVRKL APNEFPHKLY VQNYTSAVPG TCLTIRKWLF TTEEEVLLND NDLAVTYFFH QAVDDVKKGY IKAEEKSYQL QKLHEQRKMV MYLNMLRTCE GYNEIIFPHC ACDSRRKGHV ITAISITHFK LHACTEEGQL ENQVIAFEWD EMQRWDTDEE GMAFCFEYAR GEKKPRWVKI FTPYFNYMHE CFERVFCELK WRKENIFQMA RSQQRDVAT Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SNX27 (Sorting Nexin 27 (SNX27))

Alternative Name: Snx27 (SNX27 Products)

Background: Sorting nexin-27,FUNCTION: Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes. {ECO:0000269|PubMed:23524343}.

Molecular Weight: 61.0 kDa

UniProt: Q3UHD6

AlphaFold: Q3UHD6