RLIM Protein (AA 1-600) (His tag)

Catalog No. ABIN7565209

Product Details

Target: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Protein Type: Recombinant

Protein Characteristics: AA 1-600

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This RLIM protein is labelled with His tag.

Purpose: Custom-made recombinant Rlim Protein expressed in mammalian cells.

Sequence: MENSDSNDKG SDQSAAQRRS QMDRLDREEA FYQFVNNLSE EDYRLMRDNN LLGTPGESTE EELLRRLQQI KEGPPPQSPD ENRAGESSDD VTNSDSIIDW LNSVRQTGNT TRSGQRGNQS WRAVSRTNPN SGDFRFSLEI NVNRNNGSQT SENESEPSTR RLSVENMESS SQRQMENSAS ESASARPSRA ERNSAEAVTE VPTTRAQRRA RSRSPEHRRT RARAERSRSP LQPTSEIPRR APTLEQSSEN EPEGSSRTRH HVTLRQQISG PELLGRGLFA ASGSRNPSQG TSSSDTGSNS ESSGSGQRPP TIVLDLQVRR VRPGEYRQRD SIASRTRSRS QAPNNTVTYE SERGGFRRTF SRSERAGVRT YVSTIRIPIR RILNTGLSET TSVAIQTMLR QIMTGFGELS YFMYSDSDSE PSASVSSRNV ERVESRNGRG SSGGGNSSGS SSSSSPSPSS SGESSESSSE MFEGSSEGGS SGPSRRDGRH RAPVTFDESG SLPFLSLAQF FLLNEDDEDQ PRGLTKEQID NLAMRSFGEN DALKTCSVCI TEYTEGNKLR KLPCSHEYHV HCIDRWLSEN STCPICRRAV LSSGNRESVV Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Alternative Name: Rlim (RLIM Products)

Background: E3 ubiquitin-protein ligase RLIM (EC 2.3.2.27) (LIM domain-interacting RING finger protein) (RING finger LIM domain-binding protein) (R-LIM) (RING finger protein 12) (RING-type E3 ubiquitin transferase RLIM),FUNCTION: E3 ubiquitin-protein ligase that acts as a negative coregulator for LIM homeodomain transcription factors by mediating the ubiquitination and subsequent degradation of LIM cofactors LDB1 and LDB2 and by mediating the recruitment the SIN3a/histone deacetylase corepressor complex. Ubiquitination and degradation of LIM cofactors LDB1 and LDB2 allows DNA-bound LIM homeodomain transcription factors to interact with other protein partners such as RLIM. Plays a role in telomere length-mediated growth suppression by mediating the ubiquitination and degradation of TERF1. By targeting ZFP42 for degradation, acts as an activator of random inactivation of X chromosome in the embryo, a stochastic process in which one X chromosome is inactivated to minimize sex-related dosage differences of X-encoded genes in somatic cells of female placental mammals. {ECO:0000269|PubMed:10431247, ECO:0000269|PubMed:11882901, ECO:0000269|PubMed:19945382, ECO:0000269|PubMed:22596162}.

Molecular Weight: 66.4 kDa

UniProt: Q9WTV7