G Protein-Coupled Receptor 132 Protein (GPR132) (DYKDDDDK Tag,Strep Tag)
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- Target See all G Protein-Coupled Receptor 132 (GPR132) Proteins
- G Protein-Coupled Receptor 132 (GPR132)
- Protein Type
- Synthetic Nanodisc
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This G Protein-Coupled Receptor 132 protein is labelled with DYKDDDDK Tag,Strep Tag.
- Application
- ELISA, Cryogenic electron microscopy (cryo-EM), Immunogen (Imm), Phage Display (PhD), Surface Plasmon Resonance (SPR)
- Purpose
- Human GPR132-Strep full length protein-synthetic nanodisc
- Top Product
- Discover our top product GPR132 Protein
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G Protein-Coupled Receptor 132 (GPR132) (AA 1-380) protein (His tag)
custom-made GPR132 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
G Protein-Coupled Receptor 132 (GPR132) (AA 1-382) protein (His tag)custom-made GPR132 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
G Protein-Coupled Receptor 132 (GPR132) (AA 1-45) protein (Fc Tag)GPR132 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
G Protein-Coupled Receptor 132 (GPR132) (AA 281-380) protein (GST tag)GPR132 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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- Comment
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Advantages:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Mammalian cell expression system ensures post- translational modifications
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- Expiry Date
- 12 months
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- Target
- G Protein-Coupled Receptor 132 (GPR132)
- Alternative Name
- GPR132 (GPR132 Products)
- Background
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G2A
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein was reported to be a receptor for lysophosphatidylcholine action, but PubMedID: 15653487 retracts this finding and instead suggests this protein to be an effector of lysophosphatidylcholine action. This protein may have proton-sensing activity and may be a receptor for oxidized free fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] - Molecular Weight
- The human full length GPR132-Strep protein has a MW of 42.5 kDa
- UniProt
- Q9UNW8
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