G Protein-Coupled Receptor 132 Protein (GPR132)
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- Target See all G Protein-Coupled Receptor 132 (GPR132) Proteins
- G Protein-Coupled Receptor 132 (GPR132)
- Protein Type
- Synthetic
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Origin
- Human
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Source
- HEK-293 Cells
- Purpose
- Human GPR132 full length protein-synthetic nanodisc
- Characteristics
- Full Length Transmembrane Proteins (synthetic Nanodisc)
- Top Product
- Discover our top product GPR132 Protein
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G Protein-Coupled Receptor 132 (GPR132) (AA 281-380) protein (GST tag)
GPR132 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
G Protein-Coupled Receptor 132 (GPR132) (AA 1-380) protein (Strep Tag)Crystallography grade GPR132 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
G Protein-Coupled Receptor 132 (GPR132) (AA 1-382) protein (Strep Tag)Crystallography grade GPR132 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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- Applications for VLPs:
- ELISA
- SPR affinity analysis
- Phage display screening
- Immunization
- Cell based assays
- CAR-T cell screening
- Protein cystal structure analysis
- Comment
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Synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied in nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0)
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- Expiry Date
- 12 months
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- Target
- G Protein-Coupled Receptor 132 (GPR132)
- Alternative Name
- GPR132 (GPR132 Products)
- Synonyms
- G2A Protein, G2a Protein, G protein-coupled receptor 132 Protein, GPR132 Protein, gpr132 Protein, Gpr132 Protein
- Background
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G2A
Description: This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein was reported to be a receptor for lysophosphatidylcholine action, but PubMedID: 15653487 retracts this finding and instead suggests this protein to be an effector of lysophosphatidylcholine action. This protein may have proton-sensing activity and may be a receptor for oxidized free fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] - Molecular Weight
- The human full length GPR132 protein has a MW of 42.5 kDa
- UniProt
- Q9UNW8
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