TPCN1 Protein (DYKDDDDK Tag,Strep Tag)

Catalog No. ABIN7596975

Product Details

Target: TPCN1 (Two Pore Segment Channel 1 (TPCN1))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TPCN1 protein is labelled with DYKDDDDK Tag,Strep Tag.

Application: ELISA, Cryogenic electron microscopy (cryo-EM), Immunogen (Imm), Phage Display (PhD), Surface Plasmon Resonance (SPR)

Purpose: Human TPC1-Strep full length protein-synthetic nanodisc

Application Details

Comment:

Advantages:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins
• Mammalian cell expression system ensures post- translational modifications

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: TPCN1 (Two Pore Segment Channel 1 (TPCN1))

Alternative Name: TPC1 (TPCN1 Products)

Background: TPC1
Voltage-gated Ca(2 ) and Na channels have 4 homologous domains, each containing 6 transmembrane segments, S1 to S6. TPCN1 is similar to these channels, but it has only 2 domains containing S1 to S6 (Ishibashi et al., 2000 [PubMed 10753632]).[supplied by OMIM, Mar 2008]

Molecular Weight: The human full length TPC1-Strep protein has a MW of 94.1 kDa

UniProt: Q9ULQ1