TPCN1 Protein (AA 1-817) (His tag)

Catalog No. ABIN7561402

This Recombinant TPCN1 protein is produced in HEK-293 Cells.

Quick Overview for TPCN1 Protein (AA 1-817) (His tag) (ABIN7561402)

Target: TPCN1 (Two Pore Segment Channel 1 (TPCN1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-817

Purification tag / Conjugate: This TPCN1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Tpcn1 Protein expressed in mammalian cells.

Sequence: MAVSLDDDVP LILTLDEAES APLPPSNSLG QEQLPSKNGG SHSIHNSQVP SLVSGADSPP SSPTGHNWEM NYQEAAIYLQ EGQNNDKFFT HPKDARALAA YLFVHNHFFY MMELLTALLL LLLSLCESPA VPVLKLHTYV HATLELFALM VVVFELCMKL RWLGFHTFVR HKRTMVKTSV LVVQFIEAIV VLVRQTSHVR VTRALRCIFL VDCRYCGGVR RNLRQIFQSL PPFMDILLLL LFFMIIFAIL GFYLFSTNPS DPYFSTLENS IVNLFVLLTT ANFPDVMMPS YSRNPWSCVF FIVYLSIELY FIMNLLLAVV FDTFNDIEKH KFKSLLLHKR TAIQHAYGLL ASQRRPAGIS YRQFEGLMRF YKPRMSARER FLTFKALNQS NTPLLSLKDF YDIYEVAALQ WKAKRNRQHW FDELPRTAFL IFKGINILVN SKAFQYFMYL VVAVNGVWIL VETFMLKGGN FTSKHVPWSY LVFLTIYGVE LFMKVAGLGP VEYLSSGWNL FDFSVTAFAF LGLLALTLNM EPFYFIVVLR PLQLLRLFKL KKRYRNVLDT MFELLPRMAS LGLTLLTFYY SFAIVGMEFF NGRLTPNCCN TSTVADAYRF INHTVGNKTK VEEGYYYLNN FDNILNSFVT LFELTVVNNW YIIMEGVTSQ TSHWSRLYFM TFYIVTMVVM TIIVAFILEA FVFRMNYSRK SQDSEVDSGI VIEKEMSKEE LMAVLELYRE ERGTSSDVTR LLDTLSQMEK YQQNSMVFLG RRSRTKSDLS LKMYQEEIQE WYEEHAREQE QQKLRGSVPG PAAQQPPGSR QRSQTVT Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TPCN1 (Two Pore Segment Channel 1 (TPCN1))

Alternative Name: Tpcn1

Background: Two pore calcium channel protein 1 (Voltage-dependent calcium channel protein TPC1),FUNCTION: Intracellular channel initially characterized as a non-selective Ca(2+)-permeable channel activated by NAADP (nicotinic acid adenine dinucleotide phosphate), it is also a voltage-gated highly-selective Na(+) channel activated directly by PI(3,5)P2 (phosphatidylinositol 3,5-bisphosphate) that senses  pH changes and confers electrical excitability to organelles (PubMed:24776928, PubMed:23394946, PubMed:23063126). Localizes to the early and recycling endosomes membranes where it plays a role in the uptake and processing of proteins and regulates organellar membrane excitability, membrane trafficking and  pH homeostasis (PubMed:28855648). Ion selectivity is not fixed but rather agonist-dependent and under defined ionic conditions, can be readily activated by both NAADP and PI(3,5)P2 (By similarity). Required for mTOR-dependent nutrient sensing (PubMed:23394946). {ECO:0000250|UniProtKB:Q8NHX9, ECO:0000269|PubMed:23063126, ECO:0000269|PubMed:23394946, ECO:0000269|PubMed:24776928, ECO:0000269|PubMed:28855648}.

Molecular Weight: 94.5 kDa

UniProt: Q9EQJ0

AlphaFold: Q9EQJ0