Download our CUT&RUN and CUT&Tag Handbook!
In early 2017 a publication from Steven Henikoff’s lab at the Fred Hutchinson Cancer Research Center described CUT&RUN. CUT&RUN combines elements of ChIP-seq and ChIC but is superior to both methods because of its exceptionally low sample input and superior signal-to-noise ratio. The method has since found wide adoption in the epigenetics field and increasingly also beyond.
antibodies-online has compiled a handbook describing the intricacies of CUT&RUN (relying on targeted MNase DNA fragmentation) and the related method CUT&Tag (using directed tagmentation instead). This handbook gives a short overview of CUT&RUN and CUT&Tag. Subsequently, detailed step-by-step protocols are provided based on Skene PJ and Henikoff S (2017) Nature Protocols and Meers MP et al. (2019) eLIFE for CUT&RUN and Kaya-Okur HS et al. (2019) Nature Communications and Kaya-Okur HS et Kaya-Okur HS et al. (2020) Nature Protocols for CUT&Tag.
Version 1.4 features:
- Advantages of CUT&RUN and CUT&Tag in comparison with other epigenetic techniques, eg. ChIP-seq
- Step-by-step protocol*Update*
- Major FAQ on practical application questions*Update*
- All reagents required in one list
SARS-CoV-2 Handbook: Table of Contents
1. CUT&RUN:
- Advantages of CUT&RUN
- Direct or indirect CUT&RUN
- Chromatin cleavage at high Ca2+/low salt concentration
- Accurate quantitation with heterologous spike-in DNA or carry-over E.coli DNA
- CUT&RUN Reagents
- Reagent Preparation
- Protocol
- Overview CUT&Tag
2. CUT&Tag:
- Advantages of CUT&Tag
- Reagents Required
- CUT&Tag Reagents
- Protocol
- Overview CUT&Tag
3. Supplementary Material:
- Recommended Antibodies for CUT&RUN and CUT&Tag
- Frequently Asked Questions
- References
More Resources for CUT&RUN and CUT&Tag
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