antibodies-online CUT&RUN and CUT&Tag Handbook

Download our CUT&RUN and CUT&Tag Handbook!

In early 2017 a publication from Steven Henikoff’s lab at the Fred Hutchinson Cancer Research Center described CUT&RUN. CUT&RUN combines elements of ChIP-seq and ChIC but is superior to both methods because of its exceptionally low sample input and superior signal-to-noise ratio. The method has since found wide adoption in the epigenetics field and increasingly also beyond.

antibodies-online has compiled a handbook describing the intricacies of CUT&RUN (relying on targeted MNase DNA fragmentation) and the related method CUT&Tag (using directed tagmentation instead). This handbook gives a short overview of CUT&RUN and CUT&Tag. Subsequently, detailed step-by-step protocols are provided based on Skene PJ and Henikoff S (2017) Nature Protocols and Meers MP et al. (2019) eLIFE for CUT&RUN and Kaya-Okur HS et al. (2019) Nature Communications and Kaya-Okur HS et Kaya-Okur HS et al. (2020) Nature Protocols for CUT&Tag.

The version of September 2022 features:

• The CUT&RUN Low Volume Urea protocol which enables in situ profiling of rare transcription factors and non-direct DNA binding cofactors
• A wide range of antibodies validated for CUT&RUN
• Major FAQ on practical application questions*Update*
• More background information on the development of epigenetic techniques alongside with a chronological visualization