antibodies-online CUT&RUN and CUT&Tag Handbook
Download our CUT&RUN and CUT&Tag Handbook!
In early 2017 the publication of Skene PJ and Henikoff S describing CUT&RUN for the first time caught our attention. The benefits in comparison to established techniques trigger a landslide in epigenetics research gaining more and more traction among researchers.
antibodies-online supports you and your experiments in this new era of epigenetics. This handbook gives a short overview of CUT&RUN and CUT&Tag. Subsequently detailed step-by-step protocols are provided based on Skene PJ and Henikoff S (2017) Nature Protocols and Meers MP et al. (2019) eLIFE for CUT&RUN and Kaya-Okur HS et al. (2019) Nature Communications and Kaya-Okur HS et Kaya-Okur HS et al. (2020) Nature Protocols for CUT&Tag.
- Advantages of CUT&RUN and CUT&Tag in comparison with other epigenetic techniques, eg. ChIP-seq
- Step-by-step protocol
- Major FAQ on practical application questions
- All reagents required in one list
SARS-CoV-2 Handbook: Table of Contents
1. CUT&RUN:
- Advantages of CUT&RUN
- Direct or indirect CUT&RUN
- Chromatin cleavage at high Ca2+/low salt concentration
- Accurate quantitation with heterologous spike-in DNA or carry-over E.coli DNA
- CUT&RUN Reagents
- Reagent Preparation
- Protocol
- Overview CUT&Tag
- Frequently Asked Questions
2. CUT&Tag:
- Advantages of CUT&Tag
- Reagents Required
- CUT&Tag Reagents
- Protocol
- Overview CUT&Tag
- Frequently Asked Questions
3. Supplementary Material:
- Recommended Antibodies for CUT&RUN and CUT&Tag
- References
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