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antibodies-online CUT&RUN and CUT&Tag Handbook

antibodies-online cutrun-cuttag Handbookantibodies-online CUT&RUN and CUT&Tag Handbook Handbook

Download our CUT&RUN and CUT&Tag Handbook!

In early 2017 the publication of Skene PJ and Henikoff S describing CUT&RUN for the first time caught our attention. The benefits in comparison to established techniques trigger a landslide in epigenetics research gaining more and more traction among researchers.

antibodies-online supports you and your experiments in this new era of epigenetics. This handbook gives a short overview of CUT&RUN and CUT&Tag. Subsequently detailed step-by-step protocols are provided based on Skene PJ and Henikoff S (2017) Nature Protocols and Meers MP et al. (2019) eLIFE for CUT&RUN and Kaya-Okur HS et al. (2019) Nature Communications and Kaya-Okur HS et Kaya-Okur HS et al. (2020) Nature Protocols for CUT&Tag.

  • Advantages of CUT&RUN and CUT&Tag in comparison with other epigenetic techniques, eg. ChIP-seq
  • Step-by-step protocol
  • Major FAQ on practical application questions
  • All reagents required in one list

SARS-CoV-2 Handbook: Table of Contents

1. CUT&RUN:

  • Advantages of CUT&RUN
  • Direct or indirect CUT&RUN
  • Chromatin cleavage at high Ca2+/low salt concentration
  • Accurate quantitation with heterologous spike-in DNA or carry-over E.coli DNA
  • CUT&RUN Reagents
  • Reagent Preparation
  • Protocol
  • Overview CUT&Tag
  • Frequently Asked Questions

2. CUT&Tag:

  • Advantages of CUT&Tag
  • Reagents Required
  • CUT&Tag Reagents
  • Protocol
  • Overview CUT&Tag
  • Frequently Asked Questions

3. Supplementary Material:

  • Recommended Antibodies for CUT&RUN and CUT&Tag
  • References


More Resources for CUT&RUN and CUT&Tag

CUT&RUN Introduction Webinar

CUT&Tag: Workflow and Epigenetic Antibodies

Julian Pampel
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