Browse our anti-DDX3X (DDX3X) Antibodies

Human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X) interaction partners

1. Study describes the structure of a DDX3X D1D2 core in complex with a 23-base pair dsRNA at pre-unwound state, revealing that two DDXs recognize a 2-turn dsRNA, each DDX mainly recognizes a single RNA strand, and conformational changes induced by ATP binding unwinds the RNA duplex in a cooperative manner.

2. Microarray analyses revealed that DDX3 is required for the expression of a set of pro-metastatic genes, including ATF4-modulated genes in an aggressive OSCC cell line. DDX3 activated translation of ATF4 and a set of its downstream targets, all of which contain upstream open reading frames (uORF)

3. showed that PeIF2alpha-mediated ATF4 mRNA translation requires DDX3 as a part of the eIF4F complex

4. The glycine-arginine domain in DDX3X mediates interaction with RNA G-quadruplexes -containing mRNAs.

5. DDX3X immunohistochemistry combined with hematoxylin and eosin staining may help differentiate atypical meningiomas from benign meningeal tumors

6. Evidence for a novel function of DDX3X in regulating expression and downstream functions of KLF4, a master negative regulator of the cell cycle in breast cancer.

7. Results suggest that DDX3 regulates epigenetic transcriptional and translational activation of p53 and colocalizes with p53 at centrosome during mitosis to ensure proper mitotic progression and genome stability, which supports the tumor-suppressive role of DDX3.

8. Data indicate that DEAD-box helicase 3 (DDX3) directly interacts with IkappaB kinase alpha (IKKalpha) and enhances its autophosphorylation and -activation.

9. DDX3X is a substrate of both HDAC6 catalytic domains.DDX3X is acetylated under specific stress conditions.

10. Mitogen-activated protein kinases and glycogen synthase kinase 3 phosphorylate Gle1A and thereby coordinate stress granule dynamics by altering DDX3 function.

11. DDX3X is required for full CK1 activity in living cells. Phosphorylation by CK1 impairs the ATPase activity of DDX3X and RNA destabilizes the DDX3X-CK1 complex.

12. the RNA helicase DDX3, is crucial in translation initiation of HIV-1 mRNA, but also fulfills an important role as RNA sensor and inducer of antiviral immunity in dendritic cells.

13. Our genetic and functional data suggest that partial loss of function of DDX3X can cause syndromic ID. The p.R79K allele affects a region of the protein outside the critical RNA helicase domain, offering a credible explanation for the observed retention of partial function, viability in hemizygous males, and lack of pathology in females

14. our study established DDX3 as a critical host interacting partner of the arenavirus nucleoprotein

15. an N-terminal conserved Nuclear Export Signal (NES) is required for export of human DDX3 from the nucleus, and identified three regions within DDX3 that can independently facilitate its nuclear import.

16. TRPV4 mediates Ca(2+) influx and nuclear accumulation of DDX3X in cells exposed to the Zika virus. Targeting of TRPV4 reduces infectivity of dengue, hepatitis C and Zika viruses. Our results highlight the role of TRPV4 in the regulation of DDX3X-dependent control of RNA metabolism and viral infectivity.

17. DDX3 regulates MTP gene expression and lipid homeostasis through interplay with HNF4 and SHP.

18. Through adopting the immunoprecipitation (IP), RNA immunoprecipitation (RIP), dual luciferase reporter assays, the authors illustrate that DDX3X could interact with Drosha/DGCR8 complex, elevate the processing activity of Drosha/DGCR8 complex on pri-miRNAs, and increase mature miRNA expression levels.

19. our study suggested that DDX3 prevents generation of cancer stem cells through epigenetically regulating a subset of tumor-suppressive miRNAs expressions, which strengthens tumor suppressor role of DDX3 in hepatocellular carcinoma.

20. this study shows that cancer-associated DDX3X mutations drive stress granule assembly and impair global translation

Mouse (Murine) DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X) interaction partners

1. Ddx3x and Ddx3y are dispensable for spermatogenesis at least in mice. Unlike human, mice have an additional Ddx3y paralog D1pas1, that has been reported to be essential for spermatogenesis. Human and mouse DDX3 related proteins have distinct differences in their functions.

2. DDX3X is a substrate of both HDAC6 catalytic domains.DDX3X-intrinsically disordered region 1 is important for the stress granules formation.

3. Data provide further in vivo evidence for an essential contribution of a non-RLR DExD/H RNA helicase to innate immunity and suggest it may contribute to sex-related differences in resistance to microbes and resilience to inflammatory disease.

4. This study demonstrated that DDX3 regulates the translation of both Prkaca and Rac1 mRNAs in neurons and therefore maintains the PKA-Rac1 signaling axis at neurite terminals for both neurite outgrowth and dendritic spine formation.

5. Targeted Ddx3x ablation in the epiblast leads to widespread apoptosis and abnormal growth, which causes embryonic lethality in the Sox2-cre/+;Ddx3x(flox)/Y mutant around E11.5.These results have uncovered that mouse Ddx3x is essential for both embryo and extraembryonic development