anti-Human Nucleolin antibody for Immunofluorescence

Recommended Nucleolin Antibody

Antigen
Nucleolin (NCL) Antibodies
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Reactivity
Human
230
62
38
12
11
11
10
10
7
7
6
5
3
1
1
1
1
1
1
1
Host
Rabbit
122
115
1
Clonality
Polyclonal
Conjugate
This Nucleolin antibody is un-conjugated
15
10
9
9
9
8
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
209
131
124
89
63
57
39
24
21
17
13
9
2
1
1
Options

Available images

Catalog No. ABIN3032071
$ 452.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
23.593369 ABIN3025707 FACS IF IHC (p) WB Mouse IgG1 NCL-902 0
23.039883 ABIN153181 ChIP EM ICC IF IHC IHC (p) IM SimWes WB Rabbit Polyclonal 19
20.593369 ABIN3032072 IF IHC ELISA WB Mouse IgG1 130CT43-8-5 0
19 ABIN6166140 FACS IF IHC ISt WB PerCP Mouse IgG1, kappa NCL-902 0
19 ABIN6166141 FACS IF IHC ISt WB PE Mouse IgG1, kappa NCL-902 0
19 ABIN6166133 FACS IF IHC ISt WB APC Mouse IgG1, kappa NCL-902 0
19 ABIN6166144 FACS IF IHC ISt WB Mouse IgG1, kappa NCL-902 0
19 ABIN3025715 FACS IF IHC (p) WB Mouse IgG 364-5-NCL-902 0
19 ABIN3025709 FACS IF IHC (p) WB Mouse IgG1 NCL-902 0
16 ABIN3025717 FACS IF IHC (p) WB Mouse IgG 364-5-NCL-902 0
14.5933695 ABIN3028890 IF ELISA WB Rabbit Ig Fraction AA 276-304 Polyclonal 0
13 ABIN6166102 FACS IF IHC (f) ISt WB PerCP Mouse IgG1, kappa 364-5 0
13 ABIN6166103 FACS IF IHC (f) ISt WB PE Mouse IgG1, kappa 364-5 0
13 ABIN6166095 FACS IF IHC (f) ISt WB APC Mouse IgG1, kappa 364-5 0
13 ABIN6166106 FACS IF IHC (f) ISt WB Mouse IgG1, kappa 364-5 0
13 ABIN5584268 FACS IF IHC (p) WB Mouse IgG1 kappa NCL-902 0
13 ABIN3025710 FACS IF IHC (p) WB Mouse IgG1, kappa 364-5 0
13 ABIN3025347 FACS IF IHC (p) WB Mouse IgG1 NPC23-2 0
13 ABIN5584269 FACS IF IHC (p) WB Mouse IgG1 kappa NCL-902 0
11.5933695 ABIN2668241 ICC IF WB Mouse IgG1 3G4B20 0

General

Antigen Nucleolin (NCL) Antibodies
Reactivity Human
(230), (62), (38), (12), (11), (11), (10), (10), (7), (7), (6), (5), (3), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(122), (115), (1)
Clonality
Polyclonal
Conjugate This Nucleolin antibody is un-conjugated
(15), (10), (9), (9), (9), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(209), (131), (124), (89), (63), (57), (39), (24), (21), (17), (13), (9), (2), (1), (1)
Supplier

Product Details anti-Nucleolin Antibody

Target Details Nucleolin Application Details Handling Images
Cross-Reactivity (Details) Expected species reactivity: Primate
Purification Antigen affinity purified
Immunogen The amino acid sequence used as immunogen for this Nucleolin antibody is found between 425-455 of the human protein.
Isotype Ig Fraction
Plasmids, Primers & others

Target Details Nucleolin

Product Details anti-Nucleolin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Nucleolin (NCL Antibody Abstract)
Background Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [Wiki]
UniProt P19338
Pathways Ribonucleoprotein Complex Subunit Organization

Application Details

Product Details anti-Nucleolin Antibody Target Details Nucleolin Handling Images back to top
Application Notes The stated application concentrations are suggested starting amounts. Due to differences in protocols and secondary/substrate sensitivity, it may be necessary to titrate the Nucleolin antibody for optimal performance.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50
Restrictions For Research Use only

Handling

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Images back to top
Format Liquid
Buffer In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment The Nucleolin antibody should be aliquoted and and stored frozen at -20°C. Avoid repeat freeze-thaws.

Images

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Nucleolin (NCL) antibody (ABIN3032071) Fluorescent confocal image of HeLa cell stained with Nucleolin antibody at 1:25. NCL ...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) antibody (ABIN3032071) Nucleolin antibody immunohistochemistry analysis in formalin fixed and paraffin embed...
Western Blotting (WB) image for anti-Nucleolin (NCL) antibody (ABIN3032071) Nucleolin antibody western blot analysis in HepG2 lysate. Predicted molecular weight ...
Western Blotting (WB) image for anti-Nucleolin (NCL) antibody (ABIN3032071) Western blot analysis of Nucleolin antibody and 293 cell lysate (2 ug/lane) either no...
Western Blotting (WB) image for anti-Nucleolin (NCL) antibody (ABIN3032071) Nucleolin antibody western blot analysis in HepG2 lysate. Predicted molecular weight ...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) antibody (ABIN3032071) Nucleolin antibody immunohistochemistry analysis in formalin fixed and paraffin embed...
 image for anti-Nucleolin (NCL) antibody (ABIN3032071) Fluorescent confocal image of HeLa cell stained with Nucleolin antibody at 1:25. NCL ...