anti-Mouse (Murine) Nucleolin antibody for ELISA

Recommended Nucleolin Antibody (supplied by: Log in to see )

Antigen
Nucleolin (NCL) Antibodies
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Reactivity
Mouse (Murine)
233
55
38
12
11
11
10
10
7
7
6
5
3
1
1
1
1
1
1
1
Host
Rabbit
125
115
1
Clonality
Polyclonal
Conjugate
This Nucleolin antibody is conjugated to Biotin
15
11
10
9
9
9
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
217
135
124
91
63
57
41
24
21
17
13
9
2
1
1
Options
Supplier
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Supplier Product No.
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Catalog No. ABIN1175618
$ 396.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2917929 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1089627 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5873227 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5873226 ELISA IHC WB Rabbit Log in to see Polyclonal 0

General

Antigen Nucleolin (NCL) Antibodies
Reactivity Mouse (Murine)
(233), (55), (38), (12), (11), (11), (10), (10), (7), (7), (6), (5), (3), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(125), (115), (1)
Clonality
Polyclonal
Conjugate This Nucleolin antibody is conjugated to Biotin
(15), (11), (10), (9), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(217), (135), (124), (91), (63), (57), (41), (24), (21), (17), (13), (9), (2), (1), (1)
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Product Details anti-Nucleolin Antibody

Target Details Nucleolin Application Details Handling Images
Specificity It has been selected for its ability to recognize NCL in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen The antibody is a rabbit polyclonal antibody raised against NCL conjugated to biotin.
Isotype IgG
Plasmids, Primers & others

Target Details Nucleolin

Product Details anti-Nucleolin Antibody Application Details Handling Images back to top
Antigen
Pathways Ribonucleoprotein Complex Subunit Organization

Application Details

Product Details anti-Nucleolin Antibody Target Details Nucleolin Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Restrictions For Research Use only

Handling

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months