anti-Mouse (Murine) Nucleolin antibody for Western Blotting

Recommended Nucleolin Antibody

Antigen
Nucleolin (NCL) Antibodies
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Reactivity
Cat (Feline), Chicken, Cow (Bovine), Gerbil, Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
230
61
37
11
11
10
9
9
7
6
5
4
3
1
1
1
1
1
1
1
Host
Mouse
121
116
1
Clonality
Monoclonal
Conjugate
This Nucleolin antibody is un-conjugated
15
10
9
9
9
8
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
209
131
125
88
63
58
38
24
21
17
13
9
2
1
1
Options

Available images

Catalog No. ABIN3025707
$ 471.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
19.89044 ABIN3025709 FACS IF IHC (p) WB Mouse IgG1 NCL-902 0
19.33695 ABIN153181 ChIP EM ICC IF IHC IHC (p) IM SimWes WB Rabbit Polyclonal 19
16.89044 ABIN2778936 IHC WB Rabbit C-Term Polyclonal 1
13.89044 ABIN2778935 IHC WB Rabbit N-Term Polyclonal 1
13.89044 ABIN2737225 IHC WB Rabbit IgG Polyclonal 0
13.89044 ABIN3048437 IHC WB Rabbit IgG Polyclonal 0
13.89044 ABIN3025347 FACS IF IHC (p) WB Mouse IgG1 NPC23-2 0
10.89044 ABIN6570190 IHC WB Rabbit IgG Polyclonal 0
10.89044 ABIN3025349 FACS IF IHC (p) WB Mouse IgG1 NPC23-2 0
7.8904395 ABIN1175616 ICC IHC WB Rabbit IgG AA 353-568 Polyclonal 0
7.8904395 ABIN3197743 IHC WB Rabbit Polyclonal 0
7.8904395 ABIN6043718 IHC WB Rabbit IgG full length Polyclonal 0
7.8904395 ABIN2668241 ICC IF WB Mouse IgG1 3G4B20 0
7.8904395 ABIN2917928 IF/ICC IHC IP WB Rabbit AA 353-568 Polyclonal 0
7 ABIN1737582 IF IHC (p) WB Rabbit AA 284-709 Polyclonal 0
4.8904395 ABIN6718112 IHC WB Rabbit Polyclonal 0
4 ABIN1386104 IF (p) IHC (p) WB Rabbit IgG Polyclonal 0
1 ABIN6290705 WB Goat IgG C-Term Polyclonal 0
1 ABIN4889383 IF IP WB Rabbit pThr84 Polyclonal 0
1 ABIN4889382 IF IP WB Rabbit pThr76 Polyclonal 0

General

Antigen Nucleolin (NCL) Antibodies
Reactivity Cat (Feline), Chicken, Cow (Bovine), Gerbil, Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(230), (61), (37), (11), (11), (10), (9), (9), (7), (6), (5), (4), (3), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(121), (116), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Nucleolin antibody is un-conjugated
(15), (10), (9), (9), (9), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(209), (131), (125), (88), (63), (58), (38), (24), (21), (17), (13), (9), (2), (1), (1)
Supplier

Product Details anti-Nucleolin Antibody

Target Details Nucleolin Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen Recombinant human protein was used as the immunogen for the Nucleolin antibody.
Clone NCL-902
Isotype IgG1
Plasmids, Primers & others

Target Details Nucleolin

Product Details anti-Nucleolin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Nucleolin (NCL Antibody Abstract)
Background Recognizes a protein of ~76  kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.
Pathways Ribonucleoprotein Complex Subunit Organization

Application Details

Product Details anti-Nucleolin Antibody Target Details Nucleolin Handling Images back to top
Application Notes Optimal dilution of the Nucleolin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,IF: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Western blot: 0.5-1 μg/mL,Prediluted format: incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Flow cytometry of human Jurkat cells. Grey: isotype control; Green: FITC-labeled; Pur...
Flow Cytometry (FACS) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Flow cytometry testing of human 293T cells. Black: cells alone; Grey: isotype control...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Nucleolin a...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Formalin-fixed, paraffin-embedded human tonsil stained with Nucleolin antibody.
Immunofluorescence (IF) image for anti-Nucleolin (NCL) antibody (ABIN3025707) HeLa cells stained with AF488 labeled Nucleolin antibody. Green: AF488-labeled Ab. Bl...
Western Blotting (WB) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Western blot of A431 cell lysate using Nucleolin antibody.
 image for anti-Nucleolin (NCL) antibody (ABIN3025707) anti-Nucleolin (NCL) antibody (Image 7)
 image for anti-Nucleolin (NCL) antibody (ABIN3025707) anti-Nucleolin (NCL) antibody (Image 8)
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Formalin-fixed, paraffin-embedded human uterus stained with Nucleolin antibody.