Donkey anti-Goat IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN101187

Supplier Product No.: 605-703-125

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Goat

Host: Donkey

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 605-703-125

Supplier: Rockland

Purpose: Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Ch GP Ham Hs Ms Rb & Rt Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Goat IgG and Goat Serum.  No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.

Characteristics: Anti-Goat IgG Peroxidase Antibody generated in donkey detects goat IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Goat IgG (H&L) was produced by repeated immunization with Goat IgG whole molecule in donkey.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Goat IgG Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:170,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Oud, Tuijnenburg, Hempel, van Vlies, Ren, Ferdinandusse, Jansen, Santer, Johannsen, Bacchelli, Alders, Li, Davies, Dupuis, Cale, Wanders, Pals, Ocaka, James, Müller, Lehmberg, Strom, Engels, Williams et al.: "Mutations in EXTL3 Cause Neuro-immuno-skeletal Dysplasia Syndrome. ..." in: American journal of human genetics, Vol. 100, Issue 2, pp. 281-296, (2017) (PubMed).

Ellison, Gearheart, Porter, Ambruso: "IFN-γ alters the expression of diverse immunity related genes in a cell culture model designed to represent maturing neutrophils." in: PLoS ONE, Vol. 12, Issue 10, pp. e0185956, (2017) (PubMed).

Miller, Finnie, Bowden, Scholz, Oh, Kok, Burrell, Trinidad, Boyle, Li: "Preclinical efficacy studies of influenza A haemagglutinin precursor loop peptides as a potential vaccine." in: The Journal of general virology, (2011) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.