Goat anti-Rat IgG (Heavy & Light Chain) Antibody (Atto 647N) - Preadsorbed

Catalog No. ABIN965007

Supplier Product No.: 612-156-120s

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: Atto 647N

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Supplier Product No.: 612-156-120s

Supplier: Rockland

Purpose: Rat IgG (H&L) Antibody ATTO 647N Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.  This antibody will react with heavy chains of rat IgG and with light chains of most rat immunoglobulins.

Characteristics: Anti-Rat IgG (H&L) conjugated to ATTO 594 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification: Rat IgG (H&L) Antibody ATTO 647N was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Rat IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Rat IgG (H&L) conjugated to ATTO 647N has been tested by dot blot and western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. Western Blot Dilution: >1:10,000 FLISA Dilution: >1:20,000 IF Microscopy Dilution: >1:5,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Meissner, Grimm, Johnston, Sutcliffe, Ng, Jefferis, Cachero, Lavis, Malkesman: "Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons." in: PLoS ONE, Vol. 13, Issue 8, pp. e0200759, (2019) (PubMed).

Dolan, Frechter, Bates, Dan, Huoviala, Roberts, Schlegel, Dhawan, Tabano, Dionne, Christoforou, Close, Sutcliffe, Giuliani, Li, Costa, Ihrke, Meissner, Bock, Aso, Rubin, Jefferis: "Neurogenetic dissection of the Drosophila lateral horn reveals major outputs, diverse behavioural functions, and interactions with the mushroom body." in: eLife, Vol. 8, (2019) (PubMed).

Turner-Evans, Wegener, Rouault, Franconville, Wolff, Seelig, Druckmann, Jayaraman: "Angular velocity integration in a fly heading circuit." in: eLife, Vol. 6, (2018) (PubMed).

Wolff, Rubin: "Neuroarchitecture of the Drosophila central complex: A catalog of nodulus and asymmetrical body neurons and a revision of the protocerebral bridge catalog." in: The Journal of comparative neurology, Vol. 526, Issue 16, pp. 2585-2611, (2018) (PubMed).

von Reyn, Nern, Williamson, Breads, Wu, Namiki, Card: "Feature Integration Drives Probabilistic Behavior in the Drosophila Escape Response." in: Neuron, Vol. 94, Issue 6, pp. 1190-1204.e6, (2017) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Anti-Rat IgG (H&L) conjugated to ATTO 647N is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.