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Unconjugated Antibodies

Unconjugated secondary antibodies refer to antibodies that are not directly labeled or attached to any reporter molecules, such as enzymes or fluorophores. They are typically raised against the species-specific immunoglobulins of the primary antibodies used in an experiment. They have some advantages over conjugated secondary antibodies, although this should always be considered depending on the experiment:

1. flexibility in fluorescent color selection:

A major advantage of using unconjugated secondary antibodies is the flexibility in choosing the fluorescent color for the experiments. Unlike conjugated secondary antibodies, which are already labeled with a specific dye or reporter molecule label, unconjugated secondary antibodies allow the researcher to conjugate the antibody as needed with a custom label that meets the specific requirements of the experimental setup. This flexibility is particularly important when different fluorescent colors are to be used, either to detect multiple proteins simultaneously or to label specific cell components or tissue structures.

2. reduction of background signals:

The use of unconjugated secondary antibodies can help reduce background signals in experiments. Antibodies can form non-specific bonds and thus contribute to increased background signal. By using unconjugated secondary antibodies of the same isotype, such problems can be minimized. This results in a higher signal-to-noise ratio and improved accuracy of results.

3. signal amplification:

Unconjugated secondary antibodies can amplify the signal in an immunoassay by binding to primary antibodies that have already bound to the target antigen. Thus, they locally increase the concentration of Fc fragments to which labeled tertiary antibodies can bind. Since multiple non-conjugated secondary antibodies can bind to a single primary antibody, this leads to signal amplification and increased sensitivity of the respective immunoassay.

Below you will find our unconjugated secondary antibodies, which you can easily browse and filter by the properties relevant to you. If you have any questions, please contact our scientific customer support.

1,230 results :

ELISA, IHC, WB, CUT&RUN, CUT&Tag Polyclonal IgG unconjugated
Pubmed 105 references Independent Validation 1 validation
Catalog No. ABIN101961
Catalog No. ABIN2862700
ELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Pubmed 1 reference
Catalog No. ABIN965357
ELISA, IHC, WB Polyclonal IgG unconjugated
Catalog No. ABIN102079
ELISA, IHC, WB, LF Polyclonal IgG unconjugated
Catalog No. ABIN102001
IHC Monoclonal MSVA-700R IgG Recombinant Antibody unconjugated
Catalog No. ABIN7477497
IHC (p), WB, FACS, IF Monoclonal IM373 IgG1 kappa unconjugated
Catalog No. ABIN3026664
ELISA, IHC, WB Polyclonal IgG unconjugated
Pubmed 1 reference
Catalog No. ABIN102624
ELISA, IHC, WB Polyclonal IgG unconjugated
Pubmed 2 references
Catalog No. ABIN102117
ELISA, IHC, WB, FM, IP, LF Polyclonal IgG unconjugated
Pubmed 3 references
Catalog No. ABIN101983
  • Type Secondary
    • Secondary
  • Target
    • IgG
    • IgM
    • IgA
    • IgA, IgG, IgM
    • Ig
    • IgG1
    • IgE
    • IgG2a
    • IgY
    • IgD
    • IgG2
    • IgG3
    • IgG2b
    • IgG2c
    • kappa Light Chain
    • Igk
    • IgG4
    • IGHG1
    • lambda
    • Ig lambda
    • IgG, IgM
    • Lambda-IgLC
    • IgG + IgA + IgM + IgD
    • IGHM
    • IGJ
    • IgA1
    • IgG Fc
    • kappa/lambda Light Chain (Kappa/Lambda-IgLC)
    • IgA Secretory Component
    • IgA1,2
    • IgA2
    • IgG1, IgG2a, IgG2b, IgG2c
    • IgG2ab
    • IGHA1
    • IGHA2
    • CD79a (CD79A)
    • Ig Light Chains
    • IgG Light Chain
    • IgG lambda
    • IgG1, IgG2a, IgG2b, IgG3
    • IgG2, IgG3
    • IgG2a(1a)
    • IgG2a(1b)
    • IgL
    • IgM Heavy Chain
    • IGHG4
    • Light Chain
    • lambda Chain
    • ALDOC
    • Bence-Jones Protein kappa
    Please use search to find more...
  • Application
    • ELISA
    • Western Blotting (WB)
    • Immunohistochemistry (IHC)
    • Immunoprecipitation (IP)
    • Immunocytochemistry (ICC)
    • Flow Cytometry (FACS)
    • Immunoelectrophoresis (IEP)
    • Immunodiffusion (ID)
    • Enzyme Immunoassay (EIA)
    • Immunoassay (IA)
    • Immunofluorescence (IF)
    • Immunohistochemistry (Frozen Sections) (IHC (fro))
    • Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    • Radial Immunodiffusion (RID)
    • Dot Blot (DB)
    • FLISA
    • Radioimmunoassay (RIA)
    • Lateral Flow (LF)
    • Ouchterlony Double Diffusion (ODD)
    • Immunofluorescence (fixed cells) (IF/ICC)
    • ELISpot
    • Functional Studies (Func)
    • Haemagglutination (H)
    • Immunochromatography (IC)
    • Standard (STD)
    • Chromatin Immunoprecipitation (ChIP)
    • Conjugation (Con)
    • Fluorescence Microscopy (FM)
    • Agglutination (AGG)
    • Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
    • Cleavage Under Targets and Tagmentation (CUT&Tag)
    • ELISA (Detection)
    • Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    • Immunohistochemistry (Acetone-fixed) (IHC (af))
    • Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
    • Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    • Labeling (Lbl)
    • Protein Assay (PrA)
  • Reactivity
    • Human
    • Mouse
    • Rat
    • Rabbit
    • Goat
    • Chicken
    • Cow
    • Guinea Pig
    • Horse
    • Dog
    • Cat
    • Sheep
    • Monkey
    • Pig
    • Golden Syrian Hamster
    • Hamster
    • Ferret
    • Donkey
    • Duck
    • Llama
    • Armenian Hamster
    • Turkey
    • Rhesus Monkey
    • Camel
    • Chimpanzee
    • Deer
    • Fish
    • Hispid Cotton Rat
    • Mammalian
    • Pigeon
    • Reptile
    • Alligator
    • Atlantic Salmon
    • Avian
    • Baboon
    • Bat
    • Cynomolgus
    • Green Monkey
    • Iberian lynx (Lynx pardinus)
    • Mustelid
    • Opossum
    • Raccoon
    • Rainbow Trout
    • Salmon
    • Salmonella
    • Tursiops truncatus
    • Wolf
    • Zebrafish (Danio rerio)
  • Cited in publications
  • Images available
  • Independent Validation
  • Preservative free only
  • Carrier free only
  • Host
    • Goat
    • Rabbit
    • Mouse
    • Sheep
    • Donkey
    • Rat
    • Pig
    • Chicken
    • Chicken egg
    • Guinea Pig
    • Alpaca
    • Cow
    • Llama
  • Clonality
    • Polyclonal
    • Monoclonal
  • Clone
    • 11-44-2
    • 187-1
    • 23G3
    • 4F4cc
    • 7A9
    • A-1
    • B33-20
    • B3506B4
    • DIG11-124-1
    • FIG10-102-8
    • G-1
    • I17-T
    • IM373
    • IVA285-1
    • JC5-1
    • JDC-12
    • L-1
    • M 2A1
    • M-4
    • M2A1
    • RM107
    • RM110
    • RM223
    • SB73a
    • SB81a
    • SB84a
    • 10A4
    • 11-26
    • 14A3
    • 14A4
    • 15D6
    • 16C7
    • 1A23
    • 1A8
    • 1F6
    • 22G1-H8
    • 2A 8F4
    • 2A8F4
    • 2A9
    • 2B 10A8
    • 2B10A8
    • 2C 8F1
    • 2C1
    • 2C8F1
    • 3D8-H7-D4
    • 3G2
    • 3H10G5F2
    • 4-00E-003
    • 48
    • 4A11
    Please use search to find more...
  • Binding Specificity
    • Heavy & Light Chain
    • Fc Region
    • Heavy Chain
    • F(ab')2 Region
    • Fab Region
    • Chain gamma
    • Chain kappa
    • Free & Bound
    • Light Chain
    • Chain lambda
    • Chain mu
    • Whole Molecule
    • Chain alpha
    • C-Term
    • Dimer
    • Joining Chain
    • Secretory Component
    • N-Term
    • Chain delta
    • Chain epsilon
    • free
    • Chain gamma 1
    • Chain gamma 2a
    • Domain CH2
    • Fc5mu Region
    • Fd Region
    • full length
    • AA 1-234
    • AA 1-469
    • AA 1-477
    • AA 150-178
    • Center
    • Chain gamma 2b
    • Hinge Region
    • Internal Region
    • AA 1-159
    • AA 1-180
    • AA 1-232
    • AA 1-236
    • AA 1-330
    • AA 1-473
    • AA 1-489
    • AA 1-521
    • AA 11-98
    • AA 111-160
    • AA 112-206
    • AA 196-245
    • AA 211-260
    • AA 222-327
    • AA 227-276
    Please use search to find more...
  • Conjugate un-conjugated
    • Un-conjugated
    • FITC
    • HRP
    • Biotin
    • Alkaline Phosphatase (AP)
    • TRITC
    • Texas Red (TR)
    • PE
    • DyLight 488
    • DyLight 550
    • DyLight 650
    • DyLight 594
    • DyLight 800
    • Cy5
    • Cy3
    • Cy5.5
    • DyLight 680
    • DyLight 405
    • CF®488A
    • Alexa Fluor 647
    • DyLight 549
    • Alexa Fluor 488
    • Alexa Fluor 594
    • DyLight 649
    • Alexa Fluor 568
    • APC
    • Alexa Fluor 680
    • Alexa Fluor 750
    • Alexa Fluor 350
    • Alexa Fluor 555
    • CF®594
    • Cy7
    • Alexa Fluor 405
    • Alexa Fluor 532
    • Alexa Fluor 633
    • Alexa Fluor 660
    • Alexa Fluor 790
    • CF®568
    • CF®640R
    • CF®543
    • CF®633
    • CF®647
    • Rhodamine
    • CF®555
    • DyLight 633
    • CF®350
    • CF®680
    • DyLight 755
    • Agarose Beads
    • DyLight 350
    • CF®405S
  • Isotype
    • IgG
    • IgG1
    • IgG2a
    • IgG2b
    • IgM
    • IgG1 kappa
    • IgY
    • IgG kappa
    • IgG2a kappa
    • IgG2b kappa
    • IgG3
    • Cocktail
    • Ig Fraction
    • IgA
  • Protein Type
    • Recombinant Antibody
  • Format
    • Liquid
    • Lyophilized
  • Fragment
    • F(ab')2 fragment
    • Fab fragment
    • Fc fragment
    • single-domain Antibody (sdAb)
  • Supplier
    • antibodies-online
    • Acris
    • USBio
    • RayBiotech
    • Abbexa
    • Cohesion Biosciences
    • Covalab
    • Assaypro
    • Cloud-Clone
    • NSJ Bioreagents
    • CUSABIO
    • ProSci
    • RevMAb Biosciences
    • QED Bioscience
    • neweramabs
    • ANTIGENES
    • Assay Biotechnology Company, Inc.
    • Biospes
    • Boster Bio
    • Enogene Biotech
    • Hytest
    • ImmunoBioscience
    • Synaptic Systems
    • Agrisera
    • Angio-Proteomie
    • AntiProt
    • Biotium
    • ITSI-Biosciences
    • NBS-C
    • PROGEN
    • Wuhan Fine biotech
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