Rat Normal Tissue Blot II

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Western Blotting (WB)
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Characteristics The rat tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different rat tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane. The rat tissues included in the blot are adrenal, cerebellum, cerebrum, small intestine, skeletal muscle, stomach, testis, and thymus.
Sample diagnosis: Normal
Samples loaded: Lanes: 1: protein markers, 2: rat rat adrenal, 3: cerebellum, 4: cerebrum, 5: small intestine, 6: skeletal muscle, 7: stomach, 8: testis, and 9: thymus
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
Application Notes For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
Restrictions For Research Use only
Handling Advice The blot can be reused after stripping.
Storage 4 °C
Storage Comment Store at 4 °C.
Supplier Images
SDS-PAGE (SDS) image for Rat Normal Tissue Blot II (ABIN2455975) Rat Normal Tissue Blot II