Browse our anti-PKC alpha (PKCa) Antibodies

Xenopus laevis Protein Kinase C, alpha (PKCa) interaction partners

1. apically localized serine/threonine kinase aPKC directly phosphorylates an N-terminal site of the cell-cycle inhibitor p27Xic1 and reduces its ability to inhibit the cyclin-dependent kinase 2, leading to shortening of G1 and S phases

2. Neural induction by Syn4 through the PKC pathway requires inhibition of PKCdelta and activation of PKCalpha.

3. The depletion of membrane PIP(2) underlies receptor-mediated inhibition of IKs and that phosphorylation by PKC of the KCNE1 subunit underlies the GqPCR-mediated channel activation.

4. Our data demonstrate isoform-specific regulation of closed-state inactivation by protein kinase C in Kv4.3

Rhesus Monkey Protein Kinase C, alpha (PKCa) interaction partners

1. Data show that PKC staining colocalizes with monkey retina expresses a retina-specific slice variant of PCP2.

Human Protein Kinase C, alpha (PKCa) interaction partners

1. Inhibition of ezrin synergizes with lapatinib in a PKCalpha-dependent fashion to inhibit proliferation and promote apoptosis in HER2-positive breast cancer cells.

2. Study has demonstrated that ARX is a novel substrate of PRKCA, with phosphorylation at serine 174, and that the c-terminal region of ARX required to bind PICK1. Binding causes a shift in PICK1 subcellular localization to the nucleus to co-locate with the ARX protein, and truncation of this C-terminal region leads to the same loss of transcriptional activation as S174A mutant.

3. This study provided evidence for the first time that PKCalpha in colon cancer cells play an anticancer action by inducing the polarization of tumor associated macrophages to M1-like phenotype in the tumor microenvironment. PKCalpha promoted IL12/GM-CSF-mediated M1 polarization through MKK3/6-P38 signaling pathway.

4. High PRKCA expression is associated with cancer.

5. The PKCalpha directly phosphorylated Ser65 of PIM-1L, which is a long isoform of PIM-1.

6. Oxygen-glucose deprivation +/- reperfusion increased blood-brain barrier permeability. Intracellular calcium levels and protein kinase C-alpha activity were also increased. Protein kinase C-alpha regulates urokinase plasminogen activator and NADPH oxidase.

7. TNF-alpha increased the enzymatic activities of urokinase and protein kinase C alpha. TNF-alpha increased matrix metalloproteinase-2 activity and blood-brain barrier leakage.

8. D463H mutation highly specific to chordoid glioma, enhances proliferation of astrocytes and tanycytes

9. Modeling of the different conformations of PRKACA-DNAJB1 Chimeric Kinase revealed no obvious steric interactions of the J-domain with the rest of the RIIbeta holoenzyme.

10. PKC activation triggers down-regulation of Kv1.3 by inducing a clathrin-mediated endocytic event that targets the channel to lysosomal-degradative compartments.

11. Protein kinase C acts as a tumor suppressor.Cancer-associated mutations in protein kinase C are generally loss-of-function mutations.[review]

12. results could not only better explain the role of PI-PLCbeta1/PKC-alpha signaling in erythropoiesis but also lead to a better comprehension of the lenalidomide effect on del(5q) MDS and pave the way to innovative, targeted therapies.

13. A characteristic di-leucine motif (SVRPLL) in the C-terminal cytoplasmic region of ATP11C becomes functional upon PKCalpha activation. Moreover, endocytosis of ATP11C is induced by Ca(2+)-signaling via Gq-coupled receptors.

14. The haplotype carrying rs9909004 influences PRKCA expression in the heart and is associated with traits linked to heart failure, potentially affecting therapy of heart failure

15. our results demonstrate that Pc-induced expression of HO-1 is mediated by the PKCA-Nrf-2/HO-1 pathway, and inhibits UVB-induced apoptotic cell death in primary skin cells.

16. Regulation of vascular smooth muscle cell calcification by syndecan-4/FGF-2/PKCalpha signalling and cross-talk with TGF-beta1.

17. this study reveals a protective role for miR-706 by blocking the oxidative stress-induced activation of PKCalpha/TAOK1. Our results further identify a major implication for miR-706 in preventing hepatic fibrogenesis and suggest that miR-706 may be a suitable molecular target for anti-fibrosis therapy.

18. We also discuss the contribution of PKC enzymes to pancreatic diseases, including insulin resistance and diabetes mellitus, as well as pancreatitis and the development and progression of pancreatic cancer

19. data provided evidence that increased Rack1-mediated upregulation of PKC kinase activity may be responsible for the development of chemoresistance in T-ALL-derived cell line potentially by reducing FEM1b and Apaf-1 level.

20. Regulation of insulin exocytosis by calcium-dependent protein kinase C in beta cells has been summarized. (Review)

Mouse (Murine) Protein Kinase C, alpha (PKCa) interaction partners

1. PKCalpha combines a measure of recent, nearby synaptic plasticity with local synaptic input, enabling complex cellular computations such as heterosynaptic facilitation of plasticity necessary for efficient hippocampus-dependent learning

2. Together, these data suggest that global deletion of PKCa results in reduced MAP due to decreased vascular contractility.

3. High Prkca expression is associated with kidney fibrosis.

4. Prkca-mediated phosphorylation of LSD1 is required for presynaptic plasticity and hippocampal learning and memory.

5. Deleting the Prkca gene, which encodes PKCalpha, reverses diabetes-induced autophagy impairment, cellular organelle stress and apoptosis, leading to an NTD reduction. PKCalpha increases the expression of miR-129-2, which is a negative regulator of autophagy. miR-129-2 represses autophagy by directly targeting PGC-1alpha, a positive regulator for mitochondrial function, which is disturbed by maternal diabetes.

6. Its pathway may promote reactive oxygen species generation in the early state Alzheimer's disease and eventually contribute to the exacerbation of pathological phenotype

7. This indicates a role for PKC in dynamically controlling the trafficking of SNAT3 transporters in astrocytes in situ.

8. Results show that de novo polarisation of the embryo at the 8-cell stage is directed by phospholipase C (PLC) and protein kinase C (PKC).

9. LAV-BPIFB4 isoform modulates eNOS signalling through Ca2+/PKC-alpha-dependent mechanism.

10. However, the mechanism that induces release of IL-6 from skeletal muscle cells remains unknown. Here we show that heat increases IL-6 in skeletal muscle cells through the transient receptor potential vannilloid 1, PKC, and cAMP response element-binding protein signal transduction pathway.

11. protein kinase Calpha (PKCalpha) gain of function mutations may promote synaptic defects in Alzheimer's disease

12. Erk5 MAP kinase is activated in response to PDGF-BB in the smooth muscle cell line MOVAS in a manner dependent on Mekk2, Mek1/2, Mek5, PI3-kinase and protein kinase C (PKC).

13. PKC-alpha plays a crucial role in the pathophysiology of peritoneal membrane dysfunction induced by peritoneal dialysis fluids, and its therapeutic inhibition might be a valuable treatment option for peritoneal dialysis patients.

14. these results identify PKCalpha and HMGB1 as important co-regulators involved in hydrogen peroxide-induced poly-ADP-ribose formation.

15. the present study demonstrates that nNOS-derived NO signaling modulated by spinal Sig-1R activation increases Nox2 activity and concomitant ROS production, which leads to a ROS-induced increase in PKC-dependent pGluN1 expression in the spinal cord dorsal horn and the development of pain hypersensitivity

16. We conclude that synaptotagmin-1 phosphorylation is an essential step in PKC-dependent potentiation of synaptic transmission, acting downstream of the two other essential DAG/PKC substrates, Munc13-1 and Munc18-1.

17. These data indicate that LPA increases CCN2 expression through the activation of PKC and PKA. Thus, the regulatory functions of the PKC and PKA pathways are implicated in the LPA-induced increase in CCN2 expression

18. Kinocilium is essential for proper localization of Lgn, as well as Gai and aPKC, suggesting that cilium function plays a role in positioning of apical proteins critical for hearing.

19. Social defeat-driven PKA activation in the basolateral amygdala is actually a compensatory rather than pathogenic response in the homeostasis.

20. We conclude that melatonin, via modulation of PKC and Ca(2+) signaling, could potentially stimulate the Nrf2-mediated antioxidant response in mouse pancreatic acinar cells.

Guinea Pig Protein Kinase C, alpha (PKCa) interaction partners

1. PKC activation can enhance hippocampal neurotransmitter release, depending on changes specific to mGluR5 and AMPA/kainate receptors.

2. Isoenzymes beta and delta of PKC have been found significantly phosphorylated, although their location was not changed as a consequence of Trichinella spiralis infection.

Cow (Bovine) Protein Kinase C, alpha (PKCa) interaction partners

1. Mechanical stress induced biphasic and directional PKCalpha translocation

2. AngII activates PKD via a mechanism involving Src family kinases and PKC, to underlie increased aldosterone production.

3. Significant changes in thin filament Ca2+-sensitivity, structure and kinetics are brought about through PKC phosphorylation of cardiac troponin T.

4. Data indicate the involvement of PKC-alpha in proMMP-2 activation and inhibition of TIMP-2 expression by NF-kappaB-MT1-MMP-dependent and -independent pathway.

5. calcium-dependent phosphorylation of argininosuccinate synthase Ser-328 is mediated by PKCalpha

6. VDAC phosphorylation is an important determinant of its interaction with dimeric tubulin.

7. Using yeast cells co-expressing the human wild-type p53 and a single mammalian PKCalpha, delta, epsilon or zeta, results showed a differential regulation of p53-mediated apoptosis by these PKC isoforms.

8. degradation of protein kinase C(alpha)in sperm capacitation is mediated by PRKA

9. Data suggest that ceramide interacts with the calcium-dependent lipid binding C2 domain of protein kinase C alpha and thereby induces translocation of the enzyme to the Golgi compartment.

10. analysis of amino acid critical for the catalytic competence and function of protein kinase Calpha

11. By regulating VEGFR2 expression and activation, PKC-epsilon expression is critical for activation of Akt and eNOS by VEGF and contributes to VEGF-stimulated Erk activation, whereas PKC-alpha has opposite effects.

12. Protein kinase C mediated inhibition of endothelial L-arginine transport is mediated by MARCKS protein

Rabbit Protein Kinase C, alpha (PKCa) interaction partners

1. High PKC alpha expression is associated with retinal neovascularization.

2. We conclude that ouabain, even at low concentrations (0.5-8.0 mum), can increase INaL and reverse INCX , and these effects may contribute to the effect of the glycoside to increase Ca(2+) transients and contractility.

3. These results suggest a complex antagonistic interplay between G(q)-activated PKC and Gbetagamma in regulation of L-VDCC, in which multiple cytosolic segments of alpha(1C) are involved.

4. Protein kinase C alpha (PKCalpha) regulates growth and invasion of endometrial cancer cells.

Pig (Porcine) Protein Kinase C, alpha (PKCa) interaction partners

1. This study examined the protein expression and spatial-temporal distribution of PKCalpha and CPI-17 in intact smooth muscle tissues.

2. Results suggest that the action of genistein on protein kinase A is mediated via adenylate cyclase, but does not appear to involve Gs protein or ICI 182780-sensitive estrogen receptor.

3. The PKCalpha, PKCbeta1, and PKCbeta2 mRNA levels tended to be lower in ischemia-reperfused than in sham-operated eyes in both the retinal arteries and the neuroretina.

4. protein level of retinal PKC-alpha is increased with maturation