IDN3 antibody

Antigen: IDN3

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN100654

Additional Information

Gene ID: BAA77335

Immunogen: This affinity-purified antibody was prepared from whole goat serum produced by repeated immunizationswith a synthetic peptide corresponding to amino acids 344-356 of Human IDN3.

Format: Liquid (sterile filtered)

Description: IDN3 (also known as Colon tumor susceptibility 2, DKFZp434L1319, FLJ13648, Mis 4, Scc-2, Delanginand Sister chromatid cohesion protein) is the mammalian homologue of Scc2 in S. cerevisiae and Mis4 in S. pombe. Inyeast, this factor was originally identified genetically as being involved in sister chromatid cohesion, subsequent workhas determined that it is not involved in cohesion directly but is required for the loading of cohesins onto chromatinduring S phase. It shares 37% sequence identity with Nipped-B gene product of Drosophila, which facilitates enhancer-promoter communication of remote enhancers. The Drosophila protein is also homologous to a family of chromosomaladherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. IDN3 is a nuclearprotein that is widely expressed and found at the highest levels in heart, skeletal muscle, fetal and adult liver, and fetaland adult kidney. The protein is also found, albeit at lower levels of expression in thymus, placenta, peripheral leukocyteand small intestine. Multiple isoforms of the protein have been reported.

Specificity: This affinity purified antibody is directed against human IDN3 protein. The product was affinitypurified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivitywith this protein from human, rat, mouse, dog, bovine and orangutan based on 100% homology for the immunogensequence. Expect reactivity with multiple splice variants of this protein. Cross reactivity with IDN3 homologues from other sources has not been determined.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect bands at 316 kDa in size corresponding to IDN3 bywestern blotting in the appropriate cell lysate or extract. Splice variants for this protein may show molecular weights of304 kDa and 122 kDa.

Concentration: 1.6 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) yearfrom date of opening.

Research Area: Chromatin and Nuclear Signaling

Restrictions: For Research Use only

Images

Western blot using antibodies-online's 1 2 Affinity Purified anti-IDN3 antibody shows detection of bands at ~315 kDa and ~125 kDa corresponding to isoforms of IDN3 (arrowheads) in mouse heart whole cell tissue extract. Approximately 35 ug of lysate was separated on a 4-8% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Reaction occurred overnight at 4

Western blot using antibodies-online's Affinity Purified anti-IDN3 antibody shows detection of a band ~300 kDa corresponding to IDN3 (arrowhead) in human kidney (lane 1) and human heart (lane 2) whole cell tissue extracts. Approximately 20 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Use HRP conjugated Rb-a-Goat IgG [H&L] MXHu (605-403-013) for detection.