Chicken anti-Human Immunoglobulin G (IgG) Antibody (Biotin)

Antigen: Immunoglobulin G (IgG)

Clonality: Polyclonal

Host: Chicken

Reactivity: Human

Conjugate: Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (IHC)

Catalog no.: ABIN101470

Additional Information

Alternative name: Immunoglobulin G

Immunogen: Human IgG, whole molecule

Format: IgG-fraction, Lyophilized

Description: Biotin Conjugated Affinity Purified anti-Human IgG [H&L] [Chicken]. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Chicken IgG molecule.

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Chicken Serum, Human IgG and Human Serum.

Application Details

Application Notes: immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose.

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, 0.01% (w/v) Sodium Azide

Storage: Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Restrictions: For Research Use only

Publications

Publications: Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).