Goat anti-Human Immunoglobulin G (IgG) Antibody (Rhodamine)

Antigen: Immunoglobulin G (IgG)

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Rhodamine

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Flow Cytometry (FACS)

Catalog no.: ABIN101542

Additional Information

Alternative name: Immunoglobulin G

Immunogen: Human IgG whole molecule

Format: Lyophilized

Application Details

Application Notes: Label: Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 3.6 moles TRITC per mole of Goat IgG. Application Advice: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibodybased fluorescent assays requiring lot-to-lot consistency.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using HumanIgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assayby immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and HumanSerum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit,Rat and Sheep Serum Proteins.

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free Preservative: 0.01% (w/v) Sodium Azide

Storage: Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (orequivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquotcontents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if notcompletely clear after standing at room temperature. This product is stable for several weeks at 4° C as anundiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Research Area: Secondary Antibodies

Restrictions: For Research Use only

Publications

Titus JA, Haugland R, Sharrow SO et al.: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: J Immunol Methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).