Donkey anti-Mouse (Murine) IgG (Heavy & Light Chain) Antibody (Rhodamine)

Details for Product No. ABIN101711
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Antigen
Epitope
Heavy & Light Chain
(3850), (1816), (1476), (1036), (776), (228), (189), (73), (38), (33), (32), (7), (5), (2), (2), (2), (2), (1), (1)
Reactivity
Mouse (Murine)
(1937), (1908), (1583), (1238), (979), (498), (435), (343), (298), (266), (266), (260), (226), (222), (122), (93), (84), (81), (36), (28), (21), (14), (8), (8), (7), (5), (5), (4), (3), (3), (2), (1), (1), (1)
Host
Donkey
(4896), (3556), (1209), (463), (420), (254), (67), (48), (25), (8), (5), (1)
Clonality
Polyclonal
Conjugate
Rhodamine
(1541), (1379), (1250), (1003), (674), (552), (413), (218), (201), (132), (128), (115), (111), (100), (97), (36), (34), (25), (21), (20), (15), (11), (10), (10), (10), (10), (10), (10), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunomicroscopy (IM), FLISA, Western Blotting (WB)
Pubmed 1 reference available
Quantity 1 mg
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Catalog No. ABIN101711
218.90 $
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Immunogen Mouse IgG whole molecule
Isotype IgG
Specificity IgG (H&L)
Characteristics Concentration Definition: by UV absorbance at 280 nm

Label: Tetramethylrhodamine isothiocyanante (TRITC)
Molecular Weight 444 daltons.
Absorption Wavelength: 550 nm.
Emission Wavelength: 570 nm.
Fluorochrome/Protein Ratio: 3.0 moles TRITC per mole of Donkey IgG.
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum.
Research Area Immunology, Secondary Antibodies
Application Notes This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Handling Advice Product is photosensitive and should be protected from light.
General Titus, Haugland, Sharrow et al.: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: Journal of immunological methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).

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