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Secondary Antibodies
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Rabbit anti-Mouse (Murine) Immunoglobulin G (IgG) Antibody (Alkaline Phosphatase)
Rabbit anti-Mouse (Murine) Immunoglobulin G (IgG) Antibody (Alkaline Phosphatase)
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (210), Mouse (Murine) (174), Rabbit (144), Rat (Rattus) (104), Goat (91), Sheep (Ovine) (76), Guinea Pig (64), Horse (Equine) (59), Cat (Feline) (57), Cow (Bovine) (55), Chicken (46), Hamster (46), Dog (Canine) (45), Pig (Porcine) (41), Donkey (22), Monkey (19), Ferret (13), Turkey (3)
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Conjugate
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FITC (204), HRP (159), Alkaline Phosphatase (AP) (152), Biotin (151), Texas Red (TR) (133), TRITC (79), Rhodamine (56), Peroxidase (POD) (43), PE (8), APC (1), Cy2 (1), Cy3 (1), Cy5 (1), DyLight 488 (1), Gold (1), SPRD (1)
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Application
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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
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| Catalog no. |
ABIN101777 |
| Quantity |
1mg (1.0 mg/ml (by UV absorbance at 280 ...) (Variants) |
| Price |
155.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Immunoglobulin G
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Immunogen
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Mouse IgG whole molecule
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Format
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Liquid (sterile filtered)
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Application Notes
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This product was prepared from monospecific antiserum by immunoaffinity
chromatography using Mouse IgG coupled to agarose beads followed by
solid phase adsorption(s) to remove any unwanted reactivities. Assay by
immunoelectrophoresis resulted in a single precipitin arc against anti-
Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Mouse IgG and
Mouse Serum.
Recommended Dilutions:
ELISA: 1:2,000 - 1:10,000
WB:1:500 - 1:2,000
IHC:1:500 - 1:2,000
OTHER: User Optimized
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Concentration
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1.0 mg/ml (by UV absorbance at 280 nm)
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Buffer
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Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0.
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Storage
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Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
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Research Area
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Secondary Antibodies
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Restrictions
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For Research Use only
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Alternatives for antigen "Immunoglobulin G (IgG)", type "Secondary Antibodies"
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Hosts
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Rabbit (509), Goat (489), Donkey (90), Mouse (45), Sheep (42), Chicken (36), Rat (9), Guinea Pig (7), Dog (1)
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Reactivities
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Human (210), Mouse (Murine) (174), Rabbit (144), Rat (Rattus) (104), Goat (91), Sheep (Ovine) (76), Guinea Pig (64), Horse (Equine) (59), Cat (Feline) (57), Cow (Bovine) (55), Chicken (46), Hamster (46), Dog (Canine) (45), Pig (Porcine) (41), Donkey (22), Monkey (19), Ferret (13), Turkey (3)
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Conjugates
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FITC (204), HRP (159), Alkaline Phosphatase (AP) (152), Biotin (151), Texas Red (TR) (133), TRITC (79), Rhodamine (56), Peroxidase (POD) (43), PE (8), APC (1), Cy2 (1), Cy3 (1), Cy5 (1), DyLight 488 (1), Gold (1), SPRD (1)
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Epitopes
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H&L (73), Fc specific (54), Heavy Chains (38), Fab (11), Whole (9), F ab specific (2)
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