Acetylated Lysine (acLys) antibody

Antigen: Acetylated Lysine

Binding Site: acLys

Clonality: Monoclonal (7F8)

Host: Mouse

Reactivity: Chicken

Conjugate: Un-conjugated

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN1042580

Additional Information

Immunogen: Acetylated keyhole limpet hemocyanin (KLH) was used as immunogen. The cell line was screened against non acetylated KLH as a control in addition to BSA and histones

Cross-Reactivity: Chicken, Cow (Bovine)

Format: Liquid

Isotype: IgG1

Clone: 7F8

Description: Myeloma/Fusion Partners: SP2/0

Specificity: Chicken, Bovine.

Comments:

CBP and p300 are large nuclear proteins that bind to many sequencespecific factors involved in cell growth and/or differentiation, including cjun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try 1:3000-1:10,000 for EIA

Purification: Protein G purified

Buffer: PBS

Restrictions: For Research Use only

Publications

Product: Bannister, Miska: "Regulation of gene expression by transcription factor acetylation." in: Cellular and molecular life sciences : CMLS, Vol. 57, Issue 8-9, pp. 1184-92, 2000 (PubMed).