Acetylated Lysine antibody

Catalog No. ABIN479096

The Mouse Monoclonal anti-Acetylated Lysine antibody is suitable to detect Acetylated Lysine. It has been validated for WB and IP.

Quick Overview for Acetylated Lysine antibody (ABIN479096)

Target: Acetylated Lysine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Acetylated Lysine antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Acetylated Lysine Antibody

Specificity: Recognizes acetylated lysine-containing proteins including histones. Of all the possible H3 and H4 acetylation sites, this antibody exhibits highest affinity for histone H4 acetylated on lysine 8 and histone H3 acetylated on lysine 14.

Purification: Protein G purified

Immunogen: Mixture of chemically acetylated antigens.

Type of Immunogen: Hapten

Isotype: IgG

Application Details

Application Notes: Approved: IP (0.5 - 2 μg/mL), WB

Usage: Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5 μg immunoprecipitates in vitro acetylated PCAF added to a 3T3 RIPA cell lysate. The immunoprecipitated PCAF was detected by subsequent immunoblot analysis using 1 μg/mL monoclonal anti-GST.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 70 % storage buffer (0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide), 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Acetylated Lysine

Target: Acetylated Lysine

Alternative Name: Acetyl-Lysine

Target Type: Chemical