PP2A C (C-Term,Leu309) antibody

Antigen: PP2A C

Binding Site: C-Term,Leu309

Clonality: Monoclonal (2A10)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN1042659

Additional Information

Immunogen: peptide (Methyl-) of human PP2A catalytic

Cross-Reactivity: Mouse (Murine), Yeast (Saccharomyces cerevisiae)

Format: Liquid

Isotype: IgG1

Clone: 2A10

Description: Myeloma/Fusion Partners: X63-Ag8.653
Synonyms: PP2A alpha antibody,PP2A C antibody,PP2A-alpha antibody,PP2AA_HUMAN antibody,PP2Ac antibody,PP2CA antibody,PPP2CA antibody,Protein phosphatase 2, catalytic subunit, alpha isoform antibody,Replication protein C antibody

Specificity: Alpha+beta isoforms of PP2A C, human, mouse, yeast (S.cerevisiae) (experimentally determined), rat, rabbit, porcine, chicken, drosophila (not tested but identical antigenic sequence and thus predicted to be recognized)

Comments:

PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline start at 1:500 for western blot

Concentration: 1mg/ml

Purification: Protein G purified antibody (from supernatant)

Buffer: PBS

Restrictions: For Research Use only

Publications

Product: Sontag, Nunbhakdi-Craig, Sontag et al.: "Protein phosphatase 2A methyltransferase links homocysteine metabolism with tau and amyloid precursor protein regulation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 11, pp. 2751-9, 2007 (PubMed).

Hombauer, Weismann, Mudrak et al.: "Generation of active protein phosphatase 2A is coupled to holoenzyme assembly." in: PLoS biology, Vol. 5, Issue 6, pp. e155, 2007 (PubMed).