Angiopoietin 2 (ANGPT2) (N-Term) antibody

Antigen: Angiopoietin 2 (ANGPT2)

Synonyms: ANG2, AGPT2, Ang2, Agpt2, Ang-2, ang2, ANGPT2, MGC159730, ANGIOPOIETIN-2, ANG-2

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN1043684

Additional Information

Alternative name: Angiopoietin 2

Gene ID: 11601

NCBI Acession: O35608.2

UniProt: O35608

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region near the N-terminus of mouse angiopoietin-2 protein, conjugated to KLH using maleimide.
Immunogen Type: Peptide

Format: Liquid

Description: Suitable for western blotting and other antibody based assays. A 1:500 dilution is recommended for western blotting. The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean, strong signal.

Synonyms: AGPT 2 antibody, Agpt2 antibody, ANG 2 antibody, ANG2 antibody, Angiopoietin 2a antibody, Angiopoietin 2B antibody, Angiopoietin2 antibody, ANGPT 2 antibody, ANGPT2 antibody, Tie2 ligand antibody.

Characteristics: Anti Antiopoietin-2 Antibody recognizes Angiopoietin-2 (Ang-2) that has importance in development of the endothelium through regulation of tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding and thus blocks receptor phosphorylation. Ang-2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Specificity: This antiserum is directed against mouse angiopoietin-2 and shows no reactivity with angiopoietin-1 from mouse-derived proteins. A 75 kDa band is detected when assayed by immunoblot. This reagent cross-reacts with human angiopoietin-2. Partially cross-reactivity is noted with human angiopoietin-1.

Comments:

Gene Name: ANG-2

Sterility: sterile filtered

Application Details

Application Notes: ELISA Dilution: 1:5,000 - 1:25,000.
Western Blot Dilution: 1:500 - 1:2,000.

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.1% (w/v) Sodium Azide

Storage: 4°C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: Expiration date is one (1) year from date of opening.

Research Area: Cardiovascular, Angiogenesis

Restrictions: For Research Use only

Images

Rabbit anti-Ang-2 was used at a 1:500 dilution to detect mouse Ang-2 in supernatants of mouse-angiopoietin-expressing endothelial cells. Lane 1 - mock endothelial cells. Lane 2 - mouse Ang-2 (clone 2-9) expressing cells. Lane 3 - mouse Ang-1 (clone 1-15) expressing cells. Lane 4 - mouse Ang-1 (clone 1-8) expressing cells. Lane 5 - wt endothelial cells. Approximately 20 µg of each lysate was loaded for 10% SDS-PAGE. Immunoprecipitation preceded reaction with primary antibody at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (611-103-122) preceded color development.

Publications

Product: Davis, Aldrich, Jones et al.: "Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning." in: Cell, Vol. 87, Issue 7, pp. 1161-9, 1997 (PubMed).