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Angiopoietin 2 (ANGPT2) (N-Term) antibody
|Synonyms||ANG2, AGPT2, Ang2, Agpt2, Ang-2, ang2, ANGPT2, MGC159730, ANGIOPOIETIN-2, ANG-2|
Human, Mouse (Murine)
Alternatives ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
|1 reference available|
|Quantity||25 µL (Variants)|
|Price||119.90 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||Angiopoietin 2|
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region near the N-terminus of mouse angiopoietin-2 protein, conjugated to KLH using maleimide.
Immunogen Type: Peptide
Suitable for western blotting and other antibody based assays. A 1:500 dilution is recommended for western blotting. The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean, strong signal.
Synonyms: AGPT 2 antibody, Agpt2 antibody, ANG 2 antibody, ANG2 antibody, Angiopoietin 2a antibody, Angiopoietin 2B antibody, Angiopoietin2 antibody, ANGPT 2 antibody, ANGPT2 antibody, Tie2 ligand antibody.
|Characteristics||Anti Antiopoietin-2 Antibody recognizes Angiopoietin-2 (Ang-2) that has importance in development of the endothelium through regulation of tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding and thus blocks receptor phosphorylation. Ang-2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.|
|Specificity||This antiserum is directed against mouse angiopoietin-2 and shows no reactivity with angiopoietin-1 from mouse-derived proteins. A 75 kDa band is detected when assayed by immunoblot. This reagent cross-reacts with human angiopoietin-2. Partially cross-reactivity is noted with human angiopoietin-1.|
Gene Name: ANG-2
ELISA Dilution: 1:5,000 - 1:25,000.
Western Blot Dilution: 1:500 - 1:2,000.
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Preservative||0.1% (w/v) Sodium Azide|
|Storage Comment||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Expiry Date||Expiration date is one (1) year from date of opening.|
|Research Area||Cardiovascular, Angiogenesis|
|Restrictions||For Research Use only|
Davis, Aldrich, Jones et al.: "Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning." in: Cell, Vol. 87, Issue 7, pp. 1161-9, 1997 (PubMed).
|Hosts||Rabbit (40), Goat (1), Mouse (1)|
|Reactivities||Human (33), Mouse (Murine) (28), Rat (Rattus) (25), Chicken (18), Dog (Canine) (18), Pig (Porcine) (18), Rabbit (1)|
|Applications||Western Blotting (WB) (26), ELISA (18), Immunofluorescence (IF) (16), Immunohistochemistry (IHC) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Biotin (4), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (23), N-Term (1)|