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Angiopoietin 1 (ANGPT1) (N-Term) antibody

Details for Product No. ABIN1043685
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Antigen
Synonyms AGP1, AGPT, ANG1, Ang1, Ang-1, 1110046O21Rik, Agpt, Agpt1, ANG-1, ang1, wu:fc76g06, angpt1, MGC80788
Epitope
N-Term
(14), (8), (7), (6), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(59), (39), (20), (18), (14), (13), (2), (1), (1)
Host
Rabbit
(77), (6), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(9), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(73), (53), (29), (16), (16), (16), (10), (2), (2), (1)
Pubmed 1 reference available
Quantity 25 µL
Options
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Catalog No. ABIN1043685
126.50 $
Plus shipping costs $45.00 and if applicable $26.40 dry ice

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide N-Q-R-R-N-P-E-N-G-G-R-R-Y-N-R-I-Q-H-G-Q corresponding to a region (aa 21-40) near the N-terminus of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Immunogen Type: Peptide
Specificity This antiserum is directed against mouse angiopoietin 1 and shows no reactivity with mouse angiopoietin 2. A 57 kDa band is detected when assayed by immunoblot. This reagent cross-reacts with human angiopoietin 1. Partially cross reactivity is noted with human angiopoietin 2.
Characteristics Anti Antiopoietin-1 Antibody recognizes Angiopoietin-1 (Ang-1) that has importance in the development of the endothelium by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has a potential fibrinogen-like domain at the carboxyl terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, Ang-1 is also expressed in the heart and liver.
Sterility Sterile filtered
Alternative Name Angiopoietin 1
Background Anti Antiopoietin-1 Antibody recognizes Angiopoietin-1 (Ang-1) that has importance in the development of the endothelium by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has a potential fibrinogen-like domain at the carboxyl terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, Ang-1 is also expressed in the heart and liver.
Synonyms: AGP 1 antibody, AGP1 antibody, AGPT antibody, ANG 1 antibody, ANG1 antibody, Angiopoietin1 antibody, ANGPT 1 antibody, ANGPT1 antibody, KIAA0003 antibody
Gene ID 11600
NCBI Accession 46048213
UniProt O08538
Research Area Cancer, Angiogenesis
Application Notes Suitable for western blotting, Immunohistochemistry (IHC) and other antibody based assays. A 1:500 dilution is recommended for western blotting. The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean and strong signal. Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights. In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight.
Comment

Gene Name: ANG1

Restrictions For Research Use only
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Expiration date is one (1) year from date of opening.
Expiry Date 12 months
Supplier Images
anti-Angiopoietin 1 (ANGPT1) (N-Term) antibody Rabbit anti-ANG1 was used at a 1:500 dilution to detect mouse Ang-1 by western blot against supernatants of mouse angiopoietin-expressing endothelial cells. Lane 1 - wt endothelial cells. Lane 2 - mouse Ang-1 (clone 1-8) expressing cells. Lane 3 - mouse Ang-1 (clone 1-15) expressing cells. Lane 4 - mouse Ang-2 (clone 2-9) expressing cells. Approximately 20 µg of each lysate was used for 10% SDS-PAGE. Immunoprecip-itation preceded the reaction with primary antibody at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (611-103-122) preceded color development.
anti-Angiopoietin 1 (ANGPT1) (N-Term) antibody (2) anti-Angiopoietin 1 (ANGPT1) (N-Term) antibody (Image 2)
Product cited in: Suri, Jones, Patan et al.: "Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis." in: Cell, Vol. 87, Issue 7, pp. 1171-80, 1997 (PubMed).

Hosts (77), (6), (4)
Reactivities (59), (39), (20), (18), (14), (13), (2), (1), (1)
Applications (73), (53), (29), (16), (16), (16), (10), (2), (2), (1)
Conjugates (9), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (14), (8), (7), (6), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN1043685
126.50 $
Plus shipping costs $45.00 and if applicable $26.40 dry ice

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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