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Interleukin 10 (IL10) antibody
|Synonyms||CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451|
Alternatives ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
|1 reference available|
|Quantity||25 µL (80 mg/mL) (Variants)|
|Price||119.90 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
The whole rabbit serum was prepared by repeated immunizations with human IL-10.
Immunogen Type: Native Protein
This antiserum has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation, immunohistochemistry and immunoblotting. The antibody recognizes hrIL-10. Reactivity in other immunoassays is unknown.
Synonyms: CSIF antibody, Cytokine Synthesis Inhibitory Factor antibody, IL 10 antibody, IL10A antibody, Interleukin 10 antibody, Interleukin10 antibody, MGC126450 antibody, MGC126451 antibody, TGIF antibody.
|Characteristics||Anti IL-10 Antibody recognizes IL-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), that is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. IL-10 is produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappaB activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract and indeed patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant interleukin 10, showing the importance of interleukin 10 for counteracting excessive immunity in the human body. Anti-IL-10 cytokine antibody is ideal for investigators involved Immunology and Signal Transduction research.|
|Specificity||The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-10 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-10 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. The antibody will not neutralize the biological activity of murine IL-10.|
Gene Name: IL-10
ELISA Dilution: 1:20,000 - 1:100,000.
Immunohistochemistry Dilution: 1:1,000 - 1:5,000.
Western Blot Dilution: 1:2,000 - 1:10,000.
|Preservative||0.01% (w/v) Sodium Azide|
|Storage Comment||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Expiry Date||Expiration date is one (1) year from date of opening.|
|Research Area||Cytokines, Immunology, Virology, Inflammation, Cancer|
|Restrictions||For Research Use only|
Said, Dupuy, Trautmann et al.: "Programmed death-1-induced interleukin-10 production by monocytes impairs CD4+ T cell activation during HIV infection." in: Nature medicine, Vol. 16, Issue 4, pp. 452-9, 2010 (PubMed).