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Details for Product No. ABIN1043703

Interleukin 10 (IL10) antibody

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Antigen
Synonyms CSIF, GVHDS, IL-10, IL10A, TGIF, Il-10, IL10X
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Neutralization (Neut), Radioimmunoassay (RIA)
Pubmed 1 reference available
Catalog no. ABIN1043703
Quantity 25 µL
Price
126.50 $   Plus shipping costs $45.00 and if applicable $26.40 dry ice
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Immunogen The whole rabbit serum was prepared by repeated immunizations with human IL-10.
Immunogen Type: NativeProtein
Specificity The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-10 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-10 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. The antibody will not neutralize the biological activity of murine IL-10.
Characteristics Anti IL-10 Antibody recognizes IL-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), that is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. IL-10 is produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappaB activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract and indeed patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant interleukin 10, showing the importance of interleukin 10 for counteracting excessive immunity in the human body. Anti-IL-10 cytokine antibody is ideal for investigators involved Immunology and Signal Transduction research.
Sterility Sterile filtered
Alternative Name IL-10
Background Anti IL-10 Antibody recognizes IL-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), that is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. IL-10 is produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract and indeed patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant interleukin 10, showing the importance of interleukin 10 for counteracting excessive immunity in the human body. Anti-IL-10 cytokine antibody is ideal for investigators involved Immunology and Signal Transduction research.
Synonyms: CSIF antibody, Cytokine Synthesis Inhibitory Factor antibody, IL 10 antibody, IL10A antibody, Interleukin 10 antibody, Interleukin10 antibody, MGC126450 antibody, MGC126451 antibody, TGIF antibody
Gene ID 3586
NCBI Accession P22301.1
UniProt P22301
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Application Notes This antiserum has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation, immunohistochemistry and immunoblotting. The antibody recognizes hrIL-10. Reactivity in other immunoassays is unknown.
Comment

Gene Name: IL-10

Restrictions For Research Use only
Format Liquid
Concentration 80 mg/mL
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Expiration date is one (1) year from date of opening.
Expiry Date 12 months
Product cited in: Said, Dupuy, Trautmann et al.: "Programmed death-1-induced interleukin-10 production by monocytes impairs CD4+ T cell activation during HIV infection." in: Nature medicine, Vol. 16, Issue 4, pp. 452-9, 2010 (PubMed).

Alternatives for antigen "Interleukin 10 (IL10)", type "Antibodies"
Hosts (161), (124), (84), (4), (3), (3)
Reactivities (230), (149), (52), (25), (12), (12), (12), (11), (4), (3), (1), (1)
Applications (252), (222), (105), (47), (38), (30), (29), (28), (19), (11), (8), (7), (7), (7), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (1), (1)
Conjugates (67), (12), (10), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Epitopes (9), (4), (4), (3), (2), (2), (2), (2), (1), (1)
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