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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Tubulin, alpha 1B (TUBA1B) (Internal Region) antibody
|Synonyms||Tuba2, MGC102558, MGC117866, MGC118411, TUBA2, TUBA8, TUBA1B, TUBA3D, TUBA3, K-ALPHA-1, tuba, tuba1, k-alpha-1, RGD1565476|
Human, Mouse (Murine), Rat (Rattus), Chicken, Cow (Bovine)
Alternatives ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
|1 reference available|
|Quantity||25 µL (1.1 mg/mL)|
|Price||119.90 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 427-441 of Human alpha Tubulin.
Immunogen Type: Peptide
Anti-Tubulin Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.
Synonyms: Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous Tubulin K-alpha-1, TUBA1B, tubulin loading control.
|Characteristics||Tubulin Loading Control Antibody recognizes microtubules which are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.|
|Specificity||Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein. The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.|
Gene Name: TUBA1B
ELISA Dilution: 1:5,000.
Immunohistochemistry Dilution: 1:500 - 1:2,000.
IF Microscopy Dilution: 1:500 - 1:2,000.
Western Blot Dilution: 1:500 - 1:3,000.
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Preservative||0.01% (w/v) Sodium Azide|
|Storage Comment||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Expiry Date||Expiration date is one (1) year from date of opening.|
|Restrictions||For Research Use only|
Hall, Cowan: "Structural features and restricted expression of a human alpha-tubulin gene." in: Nucleic acids research, Vol. 13, Issue 1, pp. 207-23, 1985 (PubMed).