Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (C-Term) antibody

Antigen: Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1)

Synonyms: RSK, HU-1, RSK1, MAPKAPK1A

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus), Horse (Equine), Cow (Bovine), Dog (Canine)

Conjugate: Un-conjugated

Application: ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Catalog no.: ABIN1043904

Additional Information

Alternative name: p90 RSK1

Gene ID: 6195

NCBI Acession: NP_001006666.1

UniProt: Q15418

Immunogen: Anti-p90 RSK1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein.
Immunogen Type: Peptide

Format: Liquid

Isotype: IgG

Description: Anti-p90 RSK1 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 90 kDa in size corresponding to p90 RSK1 protein is expected in the appropriate cell lysate or extract.

Synonyms: p90 RSK1, p90-RSK 1, p90RSK1, p90S6K, Ribosomal S6 Kinase 1, RSK1, S6K-alpha 1, 90 kDa Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, MAPKAPK1A, MAPKAP kinase 1a, MAPKAPK-1a.

Characteristics: Ribosomal S6 Kinase 1 (RSK1) is an immediate downstream effector of mitogen activated protein kinases and therefore promotes cell proliferation and survival. It has serine/threonine kinase activity and may play a role in mediating the growth-factor and stress-induced activation of the transcription factor CREB. The C-terminal region of RSK1 is reported to be an ERK docking site, where serine 732 phosphorylation status is critical for RSK1 activation. When serine 732 is not phosphorylated, ERK1/2 binds to the ERK docking site of RSK1, and upon stimulation, activates RSK1. The activated RSK1 then autophosphorylates serine 732, leading to the dissociation of ERK from RSK1 and termination of activation by ERK. RSK1 is studied in cancer research and is known to inactive tumor supressor complexes and death kinases.

Specificity: Anti-p90 RSK1 was affinity purified from monospecific antiserum by immunoaffinity chromatography using phosphorylated peptide coupled to agarose beads followed by solid phase adsorption against non phosphorylated peptide. This antibody is specific for human p90 RSK1 protein. A BLAST analysis was used to suggest cross reactivity with p90 RSK1 from human, rat, mouse, horse, bovine, and dog based on 100% homology with the immunizing sequence. Cross reactivity with p90 RSK1 pS732 from other sources has not been determined.

Comments:

Gene Name: RPS6KA1

Sterility: sterile filtered

Application Details

Application Notes: ELISA Dilution: 1:5000-1:50,000.
Western Blot Dilution: 1µg/mL.

Concentration: 1.51 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

Western Blot of Rabbit anti-p90 RSK1 antibody. Lane 1: unstimulated HEK293T cell lysates. Lane 2: EGF stimulated HEK293T cell lysates. Load: 35 µg per lane. Primary antibody: p90 RSK1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: peroxidase conjugated secondary antibody and ECL. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 90 kDa, ~90 kDa for p90 RSK1. Other band(s): none.

Publications

Product: Chaturvedi, Poppleton, Stringfield et al.: "Subcellular localization and biological actions of activated RSK1 are determined by its interactions with subunits of cyclic AMP-dependent protein kinase." in: Molecular and cellular biology, Vol. 26, Issue 12, pp. 4586-600, 2006 (PubMed).